Isolation and characterization of Df(2R)BSC863
Kim Cook and Kevin Cook
Bloomington Stock Center
Indiana University
Df(2R)BSC863 was isolated as a FLP recombinase-induced recombination event involving P{XP}d03340 and PBac{WH}f00012. The deletion was isolated as a chromosome lacking miniwhite markers in progeny of P{hsFLP}1, y1 w1118; P{XP}d03340/PBac{WH}f00012 males crossed to w1118; P{hs-hid}2, wgSp-1/SM6a females. These males were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). The recombination event generated the genetic element P+PBac{XP5.WH5}BSC863 from the segment of P{XP}d03340 to the left of its FRT site and the segment of PBac{WH}f00012 to the right of its FRT site. The breakpoints of Df(2R)BSC863 predicted from the Release 5 genomic coordinates of the transposable element insertion sites are  2R:18685826 ;18889174 and the cytological breakpoints predicted from these coordinates are 59B1;59C1. Df(2R)BSC863 failed to complement Nup21410444 and Df(2R)BSC599. Df(2R)BSC863 heterozygotes have a Minute phenotype from deletion of RpL23. In addition, the presence of a deletion was confirmed cytologically, though the breakpoints were not analyzed in detail.