Isolation and characterization of Df(3R)BSC874
Kim Cook, Stacey Christensen and Kevin Cook
Bloomington Stock Center
Indiana University
Df(3R)BSC874 was isolated as a FLP recombinase-induced recombination event involving P{XP}d01701 and PBac{WH}CG14509f04904. The deletion was isolated as a chromosome carrying two copies of the miniwhite marker in progeny of w1118; P{hs-hid}3, Dr1/TM6C, Sb1 cu1 females crossed to P{hsFLP}1, y1 w1118; P{XP}d01701/PBac{WH}CG14509f04904 males. The males were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBac{XP3.WH3}BSC874 from the segment of P{XP}d01701 to the left of its FRT site and the segment of PBac{WH}CG14509f04904 to the right of its FRT site. Exelixis, Inc. determined the insertion site of P{XP}d01701 to be Release 3 genomic coordinate 24490117 on chromosome arm 3R, which corresponds to Release 5 coordinate  3R:24500751 . The predicted cytology of both coordinates is 98E1. PBac{WH}CG14509f04904 maps to Release 5 coordinate  3R:25017393  with predicted cytology 99A1. Consequently, the breakpoints of Df(3R)BSC874 predicted from the Release 5 genomic coordinates of the transposable element insertion sites are  3R:24500751 ;25017393 and the cytological breakpoints predicted from these coordinates are 98E1;99A1. Df(3R)BSC874 failed to complement Df(3R)BSC789 and Df(3R)BSC806.