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Citation
Wang, L., Lam, G., Thummel, C.S. (2010). Med24 and Mdh2 are required for Drosophila larval salivary gland cell death.  Dev. Dyn. 239(3): 954--964.
FlyBase ID
FBrf0210136
Publication Type
Research paper
Abstract

The steroid hormone ecdysone triggers the rapid destruction of larval tissues through transcriptional cascades that culminate in rpr and hid expression and caspase activation. Here, we show that mutations in Mdh2 and Med24 block caspase cleavage and larval salivary gland cell death. Mdh2 encodes a predicted malate dehydrogenase that localizes to mitochondria. Consistent with this proposed function, Mdh2 mutants have significantly lower levels of ATP and accumulate late-stage citric acid cycle intermediates, suggesting that the cell death defects arise from a deficit in energy production. Med24 encodes a component of the Mediator transcriptional coactivator complex. Unexpectedly, however, expression of the key death regulator genes is normal in Med24 mutant salivary glands. This study identifies novel mechanisms for controlling the destruction of larval tissues during Drosophila metamorphosis and provides new directions for our understanding of steroid-triggered programmed cell death.

PubMed ID
PubMed Central ID
PMC2945606 (PMC) (EuropePMC)
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Secondary IDs
    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    Dev. Dyn.
    Title
    Developmental Dynamics
    Publication Year
    1992-
    ISBN/ISSN
    1058-8388
    Data From Reference
    Aberrations (2)
    Alleles (8)
    Genes (13)
    Polypeptides (1)
    Natural transposons (1)
    Experimental Tools (2)
    Transgenic Constructs (5)