Reference Report

Reference
Citation	Fergestad, T., Sale, H., Bostwick, B., Schaffer, A., Ho, L., Robertson, G.A., Ganetzky, B. (2010). A Drosophila behavioral mutant, down and out (dao), is defective in an essential regulator of Erg potassium channels. Proc. Natl. Acad. Sci. U.S.A. 107(12): 5617--5621. (Export to RIS)
FlyBase ID	FBrf0210402
Publication Type	Research paper
PubMed ID	20212103
PubMed Abstract	To signal properly, excitable cells must establish and maintain the correct balance of various types of ion channels that increase or decrease membrane excitability. The mechanisms by which this balance is regulated remain largely unknown. Here, we describe a regulatory mechanism uncovered by a Drosophila behavioral mutant, down and out (dao). At elevated temperatures, dao loss-of-function mutants exhibit seizures associated with spontaneous bursts of neural activity. This phenotype closely resembles that of seizure mutations, which impair activity of ether-a-go-go-related gene (Erg)-type potassium channels. Conversely, neural over-expression of wild-type Dao confers dominant temperature-sensitive paralysis with kinetics reminiscent of paralytic sodium-channel mutants. The over-expression phenotype of dao is suppressed in a seizure mutant background, suggesting that Dao acts by an effect on Erg channels. In support of this hypothesis, functional expression of Erg channels in a heterologous system is dependent on the presence of Dao. These results indicate that Dao has an important role in establishing the proper level of neuronal membrane excitability by regulating functional expression of Erg channels.
DOI	10.1073/pnas.1001494107
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Proc. Natl. Acad. Sci. U.S.A.
Title	Proceedings of the National Academy of Sciences of the United States of America
Publication Year	1915-
ISBN/ISSN	0027-8424
Data from Reference
Aberrations (2)
	Df(2L)do1 Df(2L)rd9
Alleles (18)
	dao^b4 dao^C7 dao^g1 dao^Scer\UAS.cFa dao^{Scer\UAS.T:Avic\GFP} dao^unspecified eag¹ l(2)SH0901^SH0901 para^lk5 para^ts1 Scer\GAL4^elav.PLu Scer\GAL4^elav-C155 Scer\GAL4^Mhc.PW Scer\GAL4^tub.PU sei¹ sei^Scer\UAS.cFa sei^{Scer\UAS.T:Avic\GFP} Sh¹⁴
Constructs (7)
	P{GAL4-elav.L} P{GAL4-Mhc.W} P{tub-GAL4.U} P{UAS-dao.F} P{UAS-dao.GFP} P{UAS-sei.F} P{UAS-sei.GFP}
Genes (15)
	brp CG4168 CG15260 CG15262 Cul-3 dao eag l(2)SH0901 para Scer\GAL4 sei Sh T:Avic\GFP yuri ZnT35C
Insertions (2)
	P{GawB}elav^C155 P{lacW}l(2)SH0901^SH0901
Natural transposons (1)
	P-element