Open Close
Reference
Citation
Kang, D.N., Jung, K.I., Kim, S.H., Jeon, S.H. (2009). Computational-Aided Identification of Genes Regulated by the Drosophila Vnd.  J. Neurogenet. 23(4): 355--365.
FlyBase ID
FBrf0210468
Publication Type
Research paper
Abstract

Neurogenesis in Drosophila melanogaster is initiated by an ordered appearance of neuroblasts arranged in three columns (medial, intermediate, and lateral) in the neuroectoderm. In each column, specific homeodomain-containing genes are expressed. The ventral nervous system defective (vnd) regulates the fate of the cells in the medial domain of the neuroectoderm. In the present study, we identified Vnd-regulated genes through computational screening. Through further screening, we selected eight genes that were downregulated in the vnd loss-of function mutation. These included zfh1, uzip, CG7687, SytIV, stau, ase, scrt, and dpn genes. Ectopic expression of vnd, using the GAL4/UAS system, caused abnormal expression of all eight genes. Further, eight genes were coexpressed with that of vnd. Reverse-transcriptase polymerase chain reaction (RT-PCR) experiments showed an enhanced expression of uzip, CG7687, SytIV, and ase. Although the other four genes did not show an enhanced expression through RT-PCR, cytochemical and genetic evidence showed that these genes were regulated by Vnd. Taken together, the results obtained from this study indicate that expression of at least four genes, uzip, CG7687, SytIV, and ase, are regulated by Vnd.

PubMed ID
PubMed Central ID
Associated Information
Comments
Associated Files
Other Information
Secondary IDs
  • FBrf0209184
Language of Publication
English
Additional Languages of Abstract
Parent Publication
Publication Type
Journal
Abbreviation
J. Neurogenet.
Title
Journal of Neurogenetics
Publication Year
1983-
ISBN/ISSN
0167-7063
Data From Reference
Genes (9)