Reference Report

Reference
Citation	Meyer, N.E., Oberegelsbacher, C., Dürr, T.D., Schäfer, A., Huber, A. (2008). An eGFP-based genetic screen for defects in light-triggered subcelluar translocation of the Drosophila photoreceptor channel TRPL. Fly 2(1): 36--46. (Export to RIS)
FlyBase ID	FBrf0210490
Publication Type	Research paper
PubMed ID	18820462
PubMed Abstract	Signaling at the plasma membrane is modulated by up- and downregulation of signaling proteins. A prominent example for this type of regulation is the Drosophila TRPL ion channel that changes its spatial distribution within the photoreceptor cell. In dark-raised flies TRPL is localized in the rhabdomeral photoreceptor membrane and it translocates to the cell body upon illumination. It has been shown that TRPL translocation depends on the activation of the phototransduction cascade and requires the presence of functional rhodopsin as well as Ca2+-influx through a second lightactivated ion channel, TRP. However, little is known about the cell biological mechanism underlying TRPL translocation. Here we describe a FRT/FLP screen designed to isolate mutants defective in TRPL internalization based on the localization of eGFP-tagged TRPL in the eyes of living flies. We mutated chromosome arms 2L, 2R and 3R and isolated 12 mutants that failed to internalize TRPL. We found that four mutants did not complement genes known to affect TRPL translocation, which are trp, ninaE and inaD. Two of the isolated mutants represent new alleles of trp and ninaE. The trp allele contains a premature stop codon after amino acid 884, whereas the ninaE allele has a mutation resulting in the substitution P193S. As determined biochemically no TRP or rhodopsin protein, respectively, was expressed in the eyes of these mutants. The absence of TRP or rhodopsin in the isolated mutants readily explains the defect in TRPL internalization and proves the feasibility of our genetic screen.
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Secondary IDs	FBrf0205556
Language of Publication	English
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Publication Type	Journal
Abbreviation	Fly
Title	Fly
Publication Year	2007-
ISBN/ISSN	1933-6934 1933-6942
Data from Reference
Alleles (29)
	bw¹ cn¹ e^s Gαq¹ inaC² inaD^T1 inaD^ttd10 l(2)cl-L3¹ l(2)cl-R11¹ l(3)cl-R3¹ ninaE¹⁷ ninaE^ttd6 sr¹ trp⁹ trp^ttd2 trp^ttd4 trpl^{ninaE.T:Avic\GFP-EGFP} ttd1¹ ttd3¹ ttd5¹ Ttd7¹ Ttd8¹ Ttd9¹ Ttd11¹ Ttd12¹ w¹¹¹⁸ y¹ y⁺ y^d2
Constructs (1)
	P{Rh1-trpl-eGFP}
Genes (23)
	bw cn e Gαq inaC inaD l(2)cl-L3 l(2)cl-R11 l(3)cl-R3 ninaE sr trp trpl ttd1 ttd3 ttd5 Ttd7 Ttd8 Ttd9 Ttd11 Ttd12 w y
Natural transposons (1)
	P-element