Reference Report

Reference
Citation	Kotova, E., Jarnik, M., Tulin, A.V. (2010). Uncoupling of the transactivation and transrepression functions of PARP1 protein. Proc. Natl. Acad. Sci. U.S.A. 107(14): 6406--6411. (Export to RIS)
FlyBase ID	FBrf0210529
Publication Type	Research paper
PubMed ID	20371698
PubMed Abstract	Poly(ADP ribose) polymerase 1 (PARP1) is a nuclear protein that regulates chromatin remodeling and transcription as well as DNA repair and genome stability pathways. Recent studies have revealed a paradoxical dual role of PARP1 protein in transcription. Specifically, although PARP1 controls transcriptional activation of a subset of genes that are heat shock- or hormone-dependent, it also directly inactivates transcription, establishes heterochromatin domains, and silences retrotransposable elements. However, the domains required for these disparate functions are currently unknown. In this paper, we report the discovery of a previously undescribed mutation in the Drosophila Parp locus. We show that the mutants express a deletion mutant of PARP1 protein with an altered DNA binding domain that carries only the second Zn-finger. We demonstrate that this alteration specifically excludes PARP1 protein from heterochromatin and makes PARP1 unable to maintain repression of retrotransposable elements. By characterizing the biological activity of this unique PARP1 mutant protein isoform, we have uncoupled the transactivation and transrepression functions of this protein.
DOI	10.1073/pnas.0914152107
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Proc. Natl. Acad. Sci. U.S.A.
Title	Proceedings of the National Academy of Sciences of the United States of America
Publication Year	1915-
ISBN/ISSN	0027-8424
Data from Reference
Alleles (12)
	Parg^27.1 Parp^{1.Scer\UAS.T:Avic\GFP-ECFP} Parp^c03256 Parp^CH1 Parp^{e.Scer\UAS.T:Avic\GFP-EGFP} Parp^{I.Scer\UAS.T:Disc\RFP} Parp^{Δ300.Scer\UAS.T:Avic\GFP-EYFP} Parp^{Δ740.Scer\UAS.T:Avic\GFP-EYFP} Parp^{Δ1302.Scer\UAS.T:Avic\GFP-EYFP} Scer\GAL4^arm.PS Scer\GAL4^G1 T:Avic\GFP^ECFP
Constructs (8)
	P{GAL4} P{GAL4-arm.S} P{UAS-Parp.1.ECFP} P{UAS-Parp.e-EGFP} P{UAS-Parp.I-DsRed} P{UAS-Parp.Δ300.EYFP} P{UAS-Parp.Δ740.EYFP} P{UAS-Parp.Δ1302.EYFP}
Genes (9)
	Hsp70Aa Hsp70Ab Hsp70Ba Hsp70Bb Hsp70Bbb Hsp70Bc Parg Parp Scer\GAL4
Insertions (3)
	P{GAL4}G1 P{PZ}Parp^CH1 PBac{PB}Parp^c03256
Natural transposons (3)
	copia gypsy P-element