Reference Report

Reference
Citation	Neisch, A.L., Speck, O., Stronach, B., Fehon, R.G. (2010). Rho1 regulates apoptosis via activation of the JNK signaling pathway at the plasma membrane. J. Cell Biol. 189(2): 311--323. (Export to RIS)
FlyBase ID	FBrf0210554
Publication Type	Research paper
PubMed ID	20404112
PubMed Abstract	Precisely controlled growth and morphogenesis of developing epithelial tissues require coordination of multiple factors, including proliferation, adhesion, cell shape, and apoptosis. RhoA, a small GTPase, is known to control epithelial morphogenesis and integrity through its ability to regulate the cytoskeleton. In this study, we examine a less well-characterized RhoA function in cell survival. We demonstrate that the Drosophila melanogaster RhoA, Rho1, promotes apoptosis independently of Rho kinase through its effects on c-Jun NH(2)-terminal kinase (JNK) signaling. In addition, Rho1 forms a complex with Slipper (Slpr), an upstream activator of the JNK pathway. Loss of Moesin (Moe), an upstream regulator of Rho1 activity, results in increased levels of Rho1 at the plasma membrane and cortical accumulation of Slpr. Together, these results suggest that Rho1 functions at the cell cortex to regulate JNK activity and implicate Rho1 and Moe in epithelial cell survival.
DOI	10.1083/jcb.200912010
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Language of Publication	English
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Publication Type	Journal
Abbreviation	J. Cell Biol.
Title	Journal of Cell Biology
Publication Year	1966-
ISBN/ISSN	0021-9525
Data from Reference
Alleles (37)
	Avic\GFP^Scer\UAS.cUa bsk^DN.Scer\UAS bsk^{dsRNA.Scer\UAS} Cdc42⁴ dia^{dsRNA.Scer\UAS} Ecol\lacZ^puc-E69 Ecol\lacZ^Scer\UAS.cUa Ecol\lacZ^W-05014 egr^{dsRNA.Scer\UAS} hep^NIG.4353R Mekk1^{dsRNA.IR.Scer\UAS} Moe^{dsRNA.327-775.Scer\UAS} Moe^G0323 Moe^{Scer\UAS.T:Hsap\MYC} Moe^{T559D.Scer\UAS.T:Hsap\MYC} Mtl^Δ Pk92B^{K618M.Scer\UAS} POSH^GD11467 POSH^{Scer\UAS.T:Zzzz\FLAG} puc^E69 puc^{Scer\UAS.P\T.T:Hsap\MYC} Rac1^J11 Rac2^Δ Rho1^72R Rho1^{C189S.Scer\UAS} Rho1^E3.10 Rho1^N19.Scer\UAS Rho1^Scer\UAS.cMa rok^CAT.Scer\UAS Scer\GAL4^dpp.blk1 Scer\GAL4^en-e16E slpr^NIG.2272R Tak1^{K46R.M.Scer\UAS} Tak1^NIG.1388R Timp^Scer\UAS.cPa wgn^{dsRNA.Scer\UAS} wnd^VDRC.cUa
Constructs (26)
	P{GAL4-dpp.blk1} P{GD11467} P{NIG.1388R} P{NIG.2272R} P{NIG.4353R} P{UAS-bsk.DN} P{UAS-bsk.IR} P{UAS-dia.RNAi} P{UAS-eiger.IR} P{UAS-GFP.U} P{UAS-lacZ.U} P{UAS-Mekk1.IR} P{UAS-Moe.IR.327-775} P{UAS-Moe.myc.T559D} P{UAS-Moe.myc} P{UAS-Pk92B.K618M} P{UAS-POSH.Flag} P{UASp-puc.MYC} P{UAS-Rho1.C189S} P{UAS-Rho1.N19} P{UAS-Rho1.Sph} P{UAS-rok.CAT} P{UAS-Tak1.K46R.M} P{UAS-Timp.P} P{UAS-wengen-IR} P{VDRC.wnd.U}
Genes (29)
	Avic\GFP bsk Cdc42 dia Ecol\lacZ egr hep LanA Mekk1 Mkk4 Mmp1 Moe Mtl Pk92B POSH puc Rac1 Rac2 Rho1 rok Scer\GAL4 shg slpr T:Zzzz\FLAG Tak1 Timp W wgn wnd
Insertions (9)
	P{A92}puc^E69 P{en2.4-GAL4}e16E P{lacW}Moe^G0323 P{NIG.1388R}1 P{NIG.2272R}1 P{NIG.4353R}2 P{NIG.4353R}3 P{PZ}W⁰⁵⁰¹⁴ P{UAS-rok.CAT}3.1
Natural transposons (1)
	P-element