Reference Report

Reference
Citation	Lamiable, O., Rabhi, M., Peronnet, F., Locker, D., Decoville, M. (2010). Rm62, a DEAD-box RNA helicase, complexes with DSP1 in Drosophila embryos. genesis 48(4): 244--253. (Export to RIS)
FlyBase ID	FBrf0210563
Publication Type	Research paper
PubMed ID	20196121
PubMed Abstract	Two main classes of proteins, Polycomb group (PcG) and Trithorax group (TrxG), play a key role in the regulation of homeotic genes. These proteins act in multimeric complexes to remodel chromatin. A third class of proteins named Enhancers of Trithorax and Polycomb (ETP) modulates the activity of TrxG and PcG, but their role remains largely unknown. We previously identified an HMGB-like protein, DSP1 (Dorsal Switch Protein 1), which was classified as an ETP. Preliminary studies have revealed that DSP1 is involved in multimeric complexes. Here we identify a DEAD-box RNA helicase, Rm62, as partner of DSP1 in a 250-kDa complex. Coimmunoprecipitation assays performed on embryo extracts indicate that DSP1 and Rm62 are associated in 3- to 12-h embryos. Furthermore, DSP1 and Rm62 colocalize on polytene chromosomes. Consistent with these results, a mutation in Rm62 enhances a null mutation of dsp1 and also mutations of trxG or PcG, suggesting that Rm62 has characteristics of an ETP. We show here for the first time that an RNA helicase is involved in the maintenance of homeotic genes.
DOI	10.1002/dvg.20609
Related Publication(s)
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Associated Information
Comments
Associated Files
Other Information
Secondary IDs
Language of Publication	English
Additional Languages of Abstract
Also Published As
Parent Publication
Publication Type	Journal
Abbreviation	genesis
Title	genesis
Publication Year	2000-
ISBN/ISSN	1526-954X 1526-968X
Data from Reference
Alleles (8)
	brm² Dsp1¹ Dsp1^{Scer\UAS.P\T.T:Zzzz\FLAG} Pc¹ Rm62⁰¹⁰⁸⁶ Scer\GAL4^da.G32 trx¹ trx^E2
Constructs (2)
	P{GAL4-da.G32} P{UASp-Dsp1.3xFLAG}
Genes (7)
	brm Dsp1 Pc Rm62 Scer\GAL4 T:Zzzz\FLAG trx
Natural transposons (1)
	P-element