Reference Report

Reference
Citation	Das Thakur, M., Feng, Y., Jagannathan, R., Seppa, M.J., Skeath, J.B., Longmore, G.D. (2010). Ajuba LIM proteins are negative regulators of the Hippo signaling pathway. Curr. Biol. 20(7): 657--662. (Export to RIS)
FlyBase ID	FBrf0210592
Publication Type	Research paper
PubMed ID	20303269
PubMed Abstract	The mammalian Ajuba LIM proteins (Ajuba, LIMD1, and WTIP) are adaptor proteins that exhibit the potential to communicate cell adhesive events with nuclear responses to remodel epithelia. Determining their role in vivo, however, has been challenging due to overlapping tissue expression and functional redundancy. Thus, we turned to Drosophila, where a single gene, CG11063 or djub, exists. Drosophila lacking the djub gene or depleted of dJub by RNA interference identify djub as an essential gene for development and a novel regulator of epithelial organ size as a component of the conserved Hippo (Hpo) pathway, which has been implicated in both tissue size control and cancer development. djub-deficient tissues were small and had decreased cell numbers as a result of increased apoptosis and decreased proliferation, due to downregulation of DIAP1 and cyclin E. This phenocopies tissues deficient for Yorkie (Yki), the downstream target of the Hippo pathway. djub genetically interacts with the Hippo pathway, and epistasis suggests that djub lies downstream of hpo. In mammalian and Drosophila cells, Ajuba LIM proteins/dJub interact with LATS/Warts (Wts) and WW45/Sav to inhibit phosphorylation of YAP/Yki. This work describes a novel role for the Ajuba LIM proteins as negative regulators of the Hippo signaling pathway.
DOI	10.1016/j.cub.2010.02.035
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Curr. Biol.
Title	Current Biology
Publication Year	1991-
ISBN/ISSN	0960-9822
Data from Reference
Aberrations (2)
	Df(1)jub^I Df(1)jub^II
Alleles (21)
	BacA\p35^Scer\UAS.cHa Ecol\lacZ^th-j5C8 hpo^{dsRNA.Scer\UAS} hpo^KC202 Hsap\LIMD1^{Scer\UAS.T:Ivir\HA1} jub^{dsRNA.C.Scer\UAS.18.1} jub^{dsRNA.C.Scer\UAS.22.5} jub^{Scer\UAS.T:Disc\RFP-mCherry} sav^shrp-3 Scer\FLP1^ey.PN Scer\GAL4^Act5C.PI Scer\GAL4^Bx-MS1096 Scer\GAL4^en-e16E Scer\GAL4^GMR.PF Scer\GAL4^unspecified Scer\GAL4^αTub84B.PL T:Disc\RFP^mCherry W^GMR.PG wts^GD1563 wts^P1 yki^Scer\UAS.cHa
Constructs (14)
	P{Act5C-GAL4} P{ey-FLP.N} P{GAL4} P{GAL4-ninaE.GMR} P{GD1563} P{GMR-hid} P{tubP-GAL4} P{UAS-jub.mCherry} P{UAS-jub.RNAi.18.1} P{UAS-jub.RNAi.22.5} P{UAS-LIMD1.HA} P{UAS-p35.H} P{UAS-RNAi-hpo.P} P{UAS-yki}
Genes (15)
	BacA\p35 CycE Ecol\lacZ hpo Hsap\LIMD1 jub sav Scer\FLP1 Scer\GAL4 T:Ivir\HA1 T:Ivir\haemagglutinin th W wts yki
Insertions (7)
	P{en2.4-GAL4}e16E P{GawB}Bx^MS1096 P{lacW}th^j5C8 P{XP}Clic^d05713 P{XP}d02874 PBac{RB}jub^e03614 PBac{RB}Nup93-1^e03640
Natural transposons (1)
	P-element