Reference Report

Reference
Citation	Usha, N., Shashidhara, L.S. (2010). Interaction between Ataxin-2 Binding Protein 1 and Cubitus-interruptus during wing development in Drosophila. Dev. Biol. 341(2): 389--399. (Export to RIS)
FlyBase ID	FBrf0210647
Publication Type	Research paper
PubMed ID	20226779
PubMed Abstract	Animal growth and development is dependent on reiterative use of key signaling pathways such as Hedgehog (Hh) pathway. It is widely believed that Cubitus-interruptus (Ci) mediates all functions of Hh pathway. Here we report that CG32062, the Drosophila homologue of Ataxin-2 Binding Protein 1 (dA2BP1), functions as a cofactor of Ci to specify intervein region between L3 and L4 veins of the adult wing. Specifically, Ci-mediated transactivation of knot/collier (kn) in this region of the developing wing imaginal disc is dependent on dA2BP1 function. Protein interaction studies and chromatin-immunoprecipiation experiments suggest that Ci helps dA2BP1 to bind kn promoter, which in turn may help Ci to activate kn expression. These results suggest a mechanism by which Ci may activate targets such as kn, which do not have classical Ci/Gli-binding sites.
DOI	10.1016/j.ydbio.2010.02.039
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Dev. Biol.
Title	Developmental Biology
Publication Year	1959-
ISBN/ISSN	0012-1606
Data from Reference
Alleles (30)
	A2bp1^{dsRNA.Scer\UAS} A2bp1^EY01049 A2bp1^GD10447 A2bp1^Scer\UAS.cUa ci^{616.NZn.Scer\UAS.T:Ivir\HA1} ci^{Ce-2.Scer\UAS.T:Ivir\HA1} ci^{GA1.Scer\UAS.T:Ivir\HA1,T:Scer\GAL4} ci^{GA2.Scer\UAS.T:Ivir\HA1,T:Scer\GAL4} ci^{NZnΔNLS.Scer\UAS.T:Ivir\HA1} ci^{Scer\UAS.T:Avic\GFP-S65T,T:Hsap\MYC} ci^{ΔC1-A.Scer\UAS.T:Ivir\HA1} ci^{ΔNC1.Scer\UAS.T:Ivir\HA1} Dcr-2^Scer\UAS.cDa dsh^Scer\UAS.cNa Ecol\lacZ^{A2bp1-EN403-lacZ} Ecol\lacZ^dpp.BS3.0 Ecol\lacZ^kn.701-1991 kn^Scer\UAS.cMa Mmus\Cd8a^{Scer\UAS.T:Avic\GFP} Scer\GAL4^{A2bp1-EN403-GAL4} Scer\GAL4^Act5C.PP Scer\GAL4^ap-md544 Scer\GAL4^bbg-C96 Scer\GAL4^bi-md653 Scer\GAL4^Bx-MS1096 Scer\GAL4^hs.PB Scer\GAL4^ptc-559.1 Scer\GAL4^{Scer\FRT.Rnor\CD2.Act5C} Scer\GAL4^tub.PU Scer\GAL4^vg.PM
Constructs (22)
	P{BS3.0} P{GAL4-Act5C(FRT.CD2).P} P{GAL4-Act5C(-FRT).P} P{GAL4-Hsp70.PB} P{GAL4-vg.M} P{GD10447} P{GFP-Ci} P{kn-lacZ.701-1991} P{tub-GAL4.U} P{UAS-A2bp1.RNAi} P{UAS-A2bp1.U} P{UAS-ci.616.NZn.HA} P{UAS-ci.Ce-2.HA} P{UAS-ci.NZnΔNLS.HA} P{UAS-ci.ΔC1-A.HA} P{UAS-ciGA1} P{UAS-ciGA2} P{UAS-ciΔNC1} P{UAS-Dcr-2.D} P{UAS-dsh.N} P{UAS-kn.M} P{UAS-mCD8::GFP.L}
Genes (17)
	A2bp1 aos BacA\p35 bs ci ct CycE Dcr-2 Dl dpp dsh Ecol\lacZ kn Mmus\Cd8a ptc rl Scer\GAL4
Insertions (8)
	P{EPgy2}A2bp1^EY01049 P{GAL4}A2bp1^EN403-GAL4 P{GawB}ap^md544 P{GawB}bbg^C96 P{GawB}bi^md653 P{GawB}Bx^MS1096 P{GawB}ptc^559.1 P{lacZ}A2bp1^EN403-lacZ
Natural transposons (1)
	P-element