Reference Report

Reference
Citation	Fang, X., Lu, Q., Emoto, K., Adler, P.N. (2010). The Drosophila Fry protein interacts with Trc and is highly mobile in vivo. BMC Dev. Biol. 10(): 40. (Export to RIS)
FlyBase ID	FBrf0210776
Publication Type	Research paper
PubMed ID	20406475
PubMed Abstract	Cell polarity is a common feature of eukaryotic cells. The NDR kinases have been found to regulate polarized growth in both animal cells and fungi. Drosophila Tricornered is an NDR kinase that is essential for the normal polarized growth of extensions of epidermal cells and for the tiling and branching of dendrites of da sensory neurons. Tricornered function requires interacting with the large Furry protein (3479 amino acid).We constructed a furry (fry) transgene and established that it rescued the lethality of fry null mutations. The encoded protein was tagged at both its amino and carboxy termini and this allowed us to demonstrate that the protein existed as an uncleaved protein in vivo. We used the C terminal GFP tag to follow the protein in vivo and found it to be highly mobile. Interestingly Fry accumulated at the distal tip of growing bristles. We established that Fry and Trc could be co-immunoprecipitated from wing discs.The mobility of Fry in both bristles and dendrites suggests that it could function in directing/mediating the intracellular transport needed for polarized growth. Our observations that full length Fry and Trc show only partial co-localization in growing bristles while an amino terminal fragment of Fry shows close to complete co-localization with Trc suggests that the interaction between these proteins is transient and regulated.
DOI	10.1186/1471-213X-10-40
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Language of Publication	English
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Publication Type	Journal
Abbreviation	BMC Dev. Biol.
Title	BMC Developmental Biology
Publication Year	2002
ISBN/ISSN	1471-213X
Data from Reference
Alleles (9)
	fry¹ fry² fry^{1637.Scer\UAS.T:Hsap\MYC} fry^{Scer\UAS.T:Hsap\MYC.T:Avic\GFP} Scer\GAL4^Act.PU Scer\GAL4^{neur-GAL4-A101} Scer\GAL4^ppk.PG Scer\GAL80^ts.αTub84B trc^{Scer\UAS.T:Avic\GFP}
Constructs (6)
	P{Act-GAL4.U} P{ppk-GAL4.G} P{tubP-GAL80^ts} P{UAS-myc-fry} P{UAS-Myc-fry-GFP} P{UAS-trc-GFP}
Genes (6)
	fry Scer\GAL4 Scer\GAL80 T:Avic\GFP T:Hsap\MYC trc
Insertions (1)
	P{GawB}neur^GAL4-A101
Natural transposons (1)
	P-element