Reference Report

Reference
Citation	Saj, A., Arziman, Z., Stempfle, D., van Belle, W., Sauder, U., Horn, T., Dürrenberger, M., Paro, R., Boutros, M., Merdes, G. (2010). A combined ex vivo and in vivo RNAi screen for notch regulators in Drosophila reveals an extensive notch interaction network. Dev. Cell 18(5): 862--876. (Export to RIS)
FlyBase ID	FBrf0210834
Publication Type	Research paper
PubMed ID	20493818
PubMed Abstract	Notch signaling plays a fundamental role in cellular differentiation and has been linked to human diseases, including cancer. We report the use of comprehensive RNAi analyses to dissect Notch regulation and its connections to cellular pathways. A cell-based RNAi screen identified 900 candidate Notch regulators on a genome-wide scale. The subsequent use of a library of transgenic Drosophila expressing RNAi constructs enabled large-scale in vivo validation and confirmed 333 of 501 tested genes as Notch regulators. Mapping the phenotypic attributes of our data on an interaction network identified another 68 relevant genes and revealed several modules of unexpected Notch regulatory activity. In particular, we note an intriguing relationship to pyruvate metabolism, which may be relevant to cancer. Our study reveals a hitherto unappreciated diversity of tissue-specific modulators impinging on Notch and opens new avenues for studying Notch regulation and function in development and disease.
DOI	10.1016/j.devcel.2010.03.013
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Secondary IDs	FBrf0210974
Language of Publication	English
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Publication Type	Journal
Abbreviation	Dev. Cell
Title	Developmental Cell
Publication Year	2001-
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Data from Reference
Alleles (36)
	Atx2^GD11562 Avic\GFP^NRE.EGFP CG5261^GD16843 CG9922^GD12573 CG11876^NIG.11876R Dcr-2^Scer\UAS.cDa Disc\RFP^{Scer\UAS.T:Myr-Src64B} E(Pc)^NIG.7776R E(z)^NIG.6502R H^GD1458 kto^NIG.8491R l(2)gd1^NIG.4713R lsn^NIG.6637R N^GD144 N^{S2.Scer\UAS.T:Hsap\APP,T:Ecol\lexA,T:Hsim\VP16} N^{Scer\UAS.T:Hsim\VP16.T:Ecol\lexA} Pc³ Pc^NIG.32443R Pgi^GD13860 Pgk^NIG.3127R ph-d⁵⁰⁴ ph-p⁵⁰⁴ ph-p^GD4480 Psn^{dsRNA.Scer\UAS.cLa} PyK^NIG.7070R Scer\GAL4^bbg-C96 Scer\GAL4^en-e16E Scer\GAL4^GMR.PF Ser^GD14453 Sfmbt^NIG.16975R Su(dx)^NIG.4244R Su(H)^NIG.3497R Trl^GD8028 TSG101^GD14295 Uba1^NIG.1782R Vps28^GD7696
Constructs (31)
	P{GAL4-ninaE.GMR} P{GD144} P{GD1458} P{GD4480} P{GD7696} P{GD8028} P{GD11562} P{GD12573} P{GD13860} P{GD14295} P{GD14453} P{GD16843} P{NIG.1782R} P{NIG.3127R} P{NIG.3497R} P{NIG.4244R} P{NIG.4713R} P{NIG.6502R} P{NIG.6637R} P{NIG.7070R} P{NIG.7776R} P{NIG.8491R} P{NIG.11876R} P{NIG.16975R} P{NIG.32443R} P{NRE-EGFP.S} P{UAS-alphaN.LV} P{UAS-Dcr-2.D} P{UAS-myr-mRFP} P{UAS-N.LV} P{UAS-Psn.RNAi}
Genes (37)
	Atx2 Avic\GFP CG5261 CG9922 CG11876 ct Dcr-2 Disc\RFP Dl E(Pc) E(z) H kto kuz l(2)gd1 lqf lsn N nct Pc Pgi Pgk ph-d ph-p Psn PyK Scer\GAL4 Ser Sfmbt shrb Su(dx) Su(H) Trl TSG101 Uba1 Vps28 wg
Insertions (2)
	P{en2.4-GAL4}e16E P{GawB}bbg^C96