Home
Reference Report
| Reference | |||
|---|---|---|---|
| Citation | Watson, G.B., Chouinard, S.W., Cook, K.R., Geng, C., Gifford, J.M., Gustafson, G.D., Hasler, J.M., Larrinua, I.M., Letherer, T.J., Mitchell, J.C., Pak, W.L., Salgado, V.L., Sparks, T.C., Stilwell, G.E. (2010). A spinosyn-sensitive Drosophila melanogaster nicotinic acetylcholine receptor identified through chemically induced target site resistance, resistance gene identification, and heterologous expression. Insect Biochem. Mol. Biol. 40(5): 376--384. (Export to RIS) | ||
| FlyBase ID | FBrf0210843 | ||
| Publication Type | Research paper | ||
| PubMed ID | 19944756 | ||
| PubMed Abstract | Strains of Drosophila melanogaster with resistance to the insecticides spinosyn A, spinosad, and spinetoram were produced by chemical mutagenesis. These spinosyn-resistant strains were not cross-resistant to other insecticides. The two strains that were initially characterized were subsequently found to have mutations in the gene encoding the nicotinic acetylcholine receptor (nAChR) subunit Dalpha6. Subsequently, additional spinosyn-resistant alleles were generated by chemical mutagenesis and were also found to have mutations in the gene encoding Dalpha6, providing convincing evidence that Dalpha6 is a target site for the spinosyns in D. melanogaster. Although a spinosyn-sensitive receptor could not be generated in Xenopus laevis oocytes simply by expressing Dalpha6 alone, co-expression of Dalpha6 with an additional nAChR subunit, Dalpha5, and the chaperone protein ric-3 resulted in an acetylcholine- and spinosyn-sensitive receptor with the pharmacological properties anticipated for a native nAChR. | ||
| DOI | 10.1016/j.ibmb.2009.11.004 | ||
| Related Publication(s) | |||
Recent Updates
|
|||
| Description |
What does this section display?
This section contains items that were added to this record for each release.
It currently only tracks new links between this FlyBase report and other
FlyBase data classes (e.g. genes, references, stocks) or controlled
vocabulary terms (e.g. GO, anatomy terms).
What does this section not display?
This section does not currently display links that were removed or gene model changes.
|
||
| Update Feed |
Click the icon below to subscribe to this FlyBase record and receive updates automatically through your
feed reader.
|
||
| FB2013_03 | |||
| FB2013_02 | |||
| All updates | Click here to see a list of all updates to this record from FB2010_08 and on. | ||
|
Associated Information
|
|||
| Comments | |||
| Associated Files | |||
|
Other Information
|
|||
| Secondary IDs | |||
| Language of Publication | English | ||
| Additional Languages of Abstract | |||
| Also Published As | |||
|
Parent Publication
|
|||
| Publication Type | Journal | ||
| Abbreviation | Insect Biochem. Mol. Biol. | ||
| Title | Insect Biochemistry and Molecular Biology | ||
| Publication Year | 1992- | ||
| ISBN/ISSN | 0965-1748 | ||
|
Data from Reference
|
|||
| Aberrations (19)
|
|||
| Alleles (17)
|
|||
| Genes (4)
|
|||