Reference Report

Reference
Citation	Cook, K., Christensen, S., Cook, K. (2010.5.7). Isolation and characterization of Df(1)BSC876. (Export to RIS)
FlyBase ID	FBrf0210856
Publication Type	Personal communication to FlyBase
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PubMed Abstract
Text of Personal Communication	Df(1)BSC876 was isolated as a FLP recombinase-induced recombination event involving PBac{WH}Kmn1^f06049 and P{XP}d09482. The deletion was isolated as a chromosome carrying two copies of the miniwhite marker in progeny of PBac{WH}Kmn1^f06049/P{XP}d09482; MKRS, P{hsFLP}86E/+ females crossed to FM7h/Dp(2;Y)G, P{hs-hid}Y males. These females were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBac{XP3.WH3}BSC876 from the segment of PBac{WH}Kmn1^f06049 to the left of its FRT site and the segment of P{XP}d09482 to the right of its FRT site. The breakpoints of Df(1)BSC876 predicted from the Release 5 genomic coordinates of the transposable element insertion sites are X:11466244;11815319 and the cytological breakpoints predicted from these coordinates are 10C7;10F7. It failed to complement m¹ and m^74f.
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Language of Publication	English
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Data from Reference
Aberrations (1)
	Df(1)BSC876
Alleles (2)
	m¹ m^74f
Constructs (1)
	P+PBac{XP3.WH3}
Genes (1)
	m
Insertions (3)
	P{XP}d09482 P+PBac{XP3.WH3}BSC876 PBac{WH}Kmn1^f06049