Reference Report

Reference
Citation	Cook, K., Cook, K. (2010.5.7). Isolation and characterization of Df(2R)BSC879. (Export to RIS)
FlyBase ID	FBrf0210859
Publication Type	Personal communication to FlyBase
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PubMed Abstract
Text of Personal Communication	Df(2R)BSC879 was isolated as a FLP recombinase-induced recombination event involving P{XP}Ef1alpha48D^d10427 and PBac{RB}CG8378^e02998. The deletion was isolated as a chromosome lacking miniwhite markers in progeny of P{hsFLP}1, y¹ w¹¹¹⁸; P{XP}Ef1alpha48D^d10427/PBac{RB}CG8378^e02998 males crossed to w¹¹¹⁸; P{hs-hid}2, wg^Sp-1/SM6a females. These males were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBac{XP5.RB3}BSC879 from the segment of P{XP}Ef1alpha48D^d10427 to the left of its FRT site and the segment of PBac{RB}CG8378^e02998 to the right of its FRT site. Its presence was verified using the PCR methods and primers described in Parks et al. with the substitution of the primer 5’-GCTTCTAAACGCTTACGCATAAACGATG-3’ for the RB3’ plus or RB3’ minus primer in the Hybrid PCR protocol in the Supplementary Methods. The breakpoints of Df(2R)BSC879 predicted from the Release 5 genomic coordinates of the transposable element insertion sites are 2R:7779605;8029867 and the cytological breakpoints predicted from these coordinates are 48C5;48E2. Df(2R)BSC879 failed to complement jeb^k05644.
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Language of Publication	English
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Data from Reference
Aberrations (2)
	Df(2R)BSC879 Df(2R)BSC880
Alleles (1)
	jeb^k05644
Constructs (1)
	P+PBac{XP5.RB3}
Genes (1)
	jeb
Insertions (3)
	P{XP}Ef1α48D^d10427 P+PBac{XP5.RB3}BSC879 PBac{RB}CG8378^e02998