Reference Report

Reference
Citation	Figeac, N., Jagla, T., Aradhya, R., Da Ponte, J.P., Jagla, K. (2010). Drosophila adult muscle precursors form a network of interconnected cells and are specified by the rhomboid-triggered EGF pathway. Development 137(12): 1965--1973. (Export to RIS)
FlyBase ID	FBrf0210887
Publication Type	Research paper
PubMed ID	20463031
PubMed Abstract	In Drosophila, a population of muscle-committed stem-like cells called adult muscle precursors (AMPs) keeps an undifferentiated and quiescent state during embryonic life. The embryonic AMPs are at the origin of all adult fly muscles and, as we demonstrate here, they express repressors of myogenic differentiation and targets of the Notch pathway known to be involved in muscle cell stemness. By targeting GFP to the AMP cell membranes, we show that AMPs are tightly associated with the peripheral nervous system and with a subset of differentiated muscles. They send long cellular processes running along the peripheral nerves and, by the end of embryogenesis, form a network of interconnected cells. Based on evidence from laser ablation experiments, the main role of these cellular extensions is to maintain correct spatial positioning of AMPs. To gain insights into mechanisms that lead to AMP cell specification, we performed a gain-of-function screen with a special focus on lateral AMPs expressing the homeobox gene ladybird. Our data show that the rhomboid-triggered EGF signalling pathway controls both the specification and the subsequent maintenance of AMP cells. This finding is supported by the identification of EGF-secreting cells in the lateral domain and the EGF-dependent regulatory modules that drive expression of the ladybird gene in lateral AMPs. Taken together, our results reveal an unsuspected capacity of embryonic AMPs to form a cell network, and shed light on the mechanisms governing their specification and maintenance.
DOI	10.1242/dev.049080
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Development
Title	Development
Publication Year	1987-
ISBN/ISSN	0950-1991
Data from Reference
Aberrations (1)
	Df(3L)H99
Alleles (24)
	aop¹ Avic\GFP^m6.PL Avic\GFP^{Scer\UAS.T:Hsap\Myr2} Ecol\lacZ^{lbe.LAMPE.ETS.mut} Ecol\lacZ^{lbe.LAMPE.lb.mut} Ecol\lacZ^lbe.LAMPE Ecol\lacZ^{lbe.LME.ETS.mut} Ecol\lacZ^{lbe.LME.Mef2.mut} Ecol\lacZ^lbe.LME Egfr^{act.B.Scer\UAS} Egfr^{DN.Scer\UAS.cBa} htl^{Scer\UAS.T:λ\cI-DD} lbe^Scer\UAS.cJa pnt^P1.Scer\UAS Ras85D^V12.Scer\UAS rho^EP3704 S^IIN Scer\GAL4^69B Scer\GAL4^how-24B Scer\GAL4^kirre.PR Scer\GAL4^m6.PF Scer\GAL4^twi.PG spi¹ W^hid-A329
Constructs (17)
	P{GAL4-twi.G} P{kirre-GAL4.R} P{lbe-lacZ.LAMPE.ETS.mut} P{lbe-lacZ.LAMPE.lb.mut} P{lbe-lacZ.LAMPE} P{lbe-lacZ.LME.ETS.mut} P{lbe-lacZ.LME.Mef2.mut} P{lbe-lacZ.LME} P{m6-GAL4.F} P{m6-GFP.L} P{UAS-Egfr.act.B} P{UAS-Egfr.DN.B} P{UAS-gapGFP} P{UAS-htl.λ\cI.M} P{UAS-lbe.J} P{UAS-pnt.P1} P{UAS-Ras85D.V12}
Genes (26)
	aop Avic\GFP ct E(spl)m6-BFM Ecol\lacZ Egfr futsch grim Him htl Kr lbe lbl Mef2 pnt Ras85D rho rl rpr S Scer\GAL4 spi twi W zfh1 βTub60D
Insertions (2)
	P{GawB}69B P{GawB}how^24B
Natural transposons (1)
	P-element