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Citation
Layton, A.T., Toyama, Y., Yang, G.Q., Edwards, G.S., Kiehart, D.P., Venakides, S. (2009). Drosophila morphogenesis: tissue force laws and the modeling of dorsal closure.  HFSP J. 3(6): 441--460.
FlyBase ID
FBrf0210902
Publication Type
Research paper
Abstract
Dorsal closure, a stage of Drosophila development, is a model system for cell sheet morphogenesis and wound healing. During closure, two flanks of epidermal tissue progressively advance to reduce the area of the eye-shaped opening in the dorsal surface, which contains amnioserosa tissue. To simulate the time evolution of the overall shape of the dorsal opening, we developed a mathematical model, in which contractility and elasticity are manifest in model force-producing elements that satisfy force-velocity relationships similar to muscle. The action of the elements is consistent with the force-producing behavior of actin and myosin in cells. The parameters that characterize the simulated embryos were optimized by reference to experimental observations on wild-type embryos and, to a lesser extent, on embryos whose amnioserosa was removed by laser surgery and on myospheroid mutant embryos. Simulations failed to reproduce the amnioserosa-removal protocol in either the elastic or the contractile limit, indicating that both elastic and contractile dynamics are essential components of the biological force-producing elements. We found it was necessary to actively upregulate forces to recapitulate both the double and single-canthus nick protocols, which did not participate in the optimization of parameters, suggesting the existence of additional key feedback mechanisms.
PubMed ID
PubMed Central ID
PMC2839815 (PMC) (EuropePMC)
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Secondary IDs
    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    HFSP J.
    Title
    HFSP Journal : frontiers of interdisciplinary research in the life sciences
    ISBN/ISSN
    1955-2068 1955-205X
    Data From Reference
    Genes (2)