Reference Report

Reference
Citation	Yan, D., Wu, Y., Yang, Y., Belenkaya, T.Y., Tang, X., Lin, X. (2010). The cell-surface proteins Dally-like and Ihog differentially regulate Hedgehog signaling strength and range during development. Development 137(12): 2033--2044. (Export to RIS)
FlyBase ID	FBrf0210906
Publication Type	Research paper
PubMed ID	20501592
PubMed Abstract	Hedgehog (Hh) acts as a morphogen in various developmental contexts to specify distinct cell fates in a concentration-dependent manner. Hh signaling is regulated by two conserved cell-surface proteins: Ig/fibronectin superfamily member Interference hedgehog (Ihog) and Dally-like (Dlp), a glypican that comprises a core protein and heparan sulfate glycosaminoglycan (GAG) chains. Here, we show in Drosophila that the Dlp core protein can interact with Hh and is essential for its function in Hh signaling. In wing discs, overexpression of Dlp increases short-range Hh signaling while reducing long-range signaling. By contrast, Ihog has biphasic activity in Hh signaling in cultured cells: low levels of Ihog increase Hh signaling, whereas high levels decrease it. In wing discs, overexpression of Ihog represses high-threshold targets, while extending the range of low-threshold targets, thus showing opposite effects to Dlp. We further show that Ihog and its family member Boi are required to maintain Hh on the cell surface. Finally, Ihog and Dlp have complementary expression patterns in discs. These data led us to propose that Dlp acts as a signaling co-receptor. However, Ihog might not act as a classic co-receptor; rather, it may act as an exchange factor by retaining Hh on the cell surface, but also compete with the receptor for Hh binding.
DOI	10.1242/dev.045740
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Secondary IDs
Language of Publication	English
Additional Languages of Abstract
Also Published As
Parent Publication
Publication Type	Journal
Abbreviation	Development
Title	Development
Publication Year	1987-
ISBN/ISSN	0950-1991
Data from Reference
Aberrations (1)
	Df(2L)ihog^Δ
Alleles (31)
	boi^{dsRNA.Scer\UAS.cIa} CG10158^Δ Con^{Scer\UAS.T:SV5\V5} dally⁸⁰ dlp^A187 dlp^{-HS.Scer\UAS.T:Avic\GFP-EGFP,T:Rnor\CD2} dlp^{-HS.Scer\UAS.T:Avic\GFP-EGFP} dlp^{-HS.Scer\UAS.T:Rnor\CD2} dlp^-HS.Scer\UAS dlp^Scer\UAS.cBa dlp^{Scer\UAS.T:Avic\GFP,T:Rnor\CD2} dlp^{Scer\UAS.T:Avic\GFP} dlp^ΔC.Scer\UAS dlp^{ΔGAG.Scer\UAS} dlp^ΔN.Scer\UAS Ecol\lacZ^dpp-10638 hh^Scer\UAS.cIa ihog^{dsRNA.Scer\UAS.cIa} ihog^Scer\UAS.cYa ihog^Δ Rab5^{S43N.Scer\UAS} Scer\GAL4^ap-md544 Scer\GAL4^Bx-MS1096 Scer\GAL4^ci.PC Scer\GAL4^prd.RG1 Scer\GAL4^ptc-559.1 Scer\GAL4^αTub84B.PL Scer\GAL80^ts.αTub84B sfl^9B4 sgl⁰⁸³¹⁰ wg^Scer\UAS.cKa
Constructs (21)
	P{ci-GAL4.C} P{GAL4-prd.F} P{tubP-GAL4} P{tubP-GAL80^ts} P{UAS-boi.RNAi} P{UAS-Con.V5} P{UAS-dlp.GFP} P{UAS-dlp.-HS.CD2} P{UAS-dlp.-HS.GFP.CD2} P{UAS-dlp.-HS.GFP} P{UAS-dlp.-HS} P{UAS-dlp.WT} P{UAS-dlp.ΔC} P{UAS-dlp.ΔGAG} P{UAS-dlp.ΔN} P{UAS-GFP-dlp-CD2} P{UAS-hh.I} P{UAS-ihog.RNAi} P{UAS-ihog.Y} P{UAS-Rab5.S43N} P{UAS-wg.K}
Genes (20)
	bap boi CG10158 ci Con dally dlp dpp Ecol\lacZ hh ihog kn ptc Rab5 Scer\GAL4 Scer\GAL80 sfl sgl T:SV5\V5 wg
Insertions (5)
	P{GawB}ap^md544 P{GawB}Bx^MS1096 P{GawB}ptc^559.1 P{PZ}dpp¹⁰⁶³⁸ P{SUPor-P}CG10158^KG05348
Natural transposons (1)
	P-element