Reference Report

Reference
Citation	Lee, J., Moon, S., Cha, Y., Chung, Y.D. (2010). Drosophila TRPN(=NOMPC) channel localizes to the distal end of mechanosensory cilia. PLoS ONE 5(6): e11012. (Export to RIS)
FlyBase ID	FBrf0210994
Publication Type	Research paper
PubMed ID	20543979
PubMed Abstract	A TRPN channel protein is essential for sensory transduction in insect mechanosensory neurons and in vertebrate hair cells. The Drosophila TRPN homolog, NOMPC, is required to generate mechanoreceptor potentials and currents in tactile bristles. NOMPC is also required, together with a TRPV channel, for transduction by chordotonal neurons of the fly's antennal ear, but the TRPN or TRPV channels have distinct roles in transduction and in regulating active antennal mechanics. The evidence suggests that NOMPC is a primary mechanotransducer channel, but its subcellular location-key for understanding its exact role in transduction-has not yet been established.Here, by immunostaining, we locate NOMPC at the tips of mechanosensory cilia in both external and chordotonal sensory neurons, as predicted for a mechanotransducer channel. In chordotonal neurons, the TRPN and TRPV channels are respectively segregated into distal and proximal ciliary zones. This zonal separation is demarcated by and requires the ciliary dilation, an intraciliary assembly of intraflagellar transport (IFT) proteins.Our results provide a strong evidence for NOMPC as a primary transduction channel in Drosophila mechansensory organs. The data also reveals a structural basis for the model of auditory chordotonal transduction in which the TRPN and TRPV channels play sequential roles in generating and amplifying the receptor potential, but have opposing roles in regulating active ciliary motility.
DOI	10.1371/journal.pone.0011012
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Language of Publication	English
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Publication Type	Journal
Abbreviation	PLoS ONE
Title	PLoS ONE
Publication Year	2006-
ISBN/ISSN	1932-6203
Data from Reference
Alleles (13)
	btv^5P1 iav^{Cterm.T:Avic\GFP-EGFP} Mmus\Cd8a^{Scer\UAS.T:Avic\GFP} nompA^T:Avic\GFP nompB^{T:Avic\GFP-EGFP} nompC¹ nompC² nompC⁴ nompC^f00642 rempA^{T:Avic\GFP-YFP.Venus} Scer\GAL4^JO15 Scer\GAL4^nan.PK Scer\GAL4^NP6250
Constructs (7)
	P{EGFP-nompB} P{iav-GFP.Cterm} P{J21.17-GAL4} P{nan-GAL4.K} P{nompA.GFP} P{rempA⁺-YFP} P{UAS-mCD8::GFP.L}
Genes (11)
	btv eys futsch iav Mmus\Cd8a nan nompA nompB nompC rempA Scer\GAL4
Insertions (1)
	P{GawB}NP6250
Polypeptides (2)
	iav^{Cterm.T:Avic\GFP-EGFP}PA nompA^T:Avic\GFPPA
Transcripts (3)
	Scer\GAL4^JO15RA Scer\GAL4^nan.PKRA Scer\GAL4^NP6250RA