Reference Report

Reference
Citation	Sato, D., Sugimura, K., Satoh, D., Uemura, T. (2010). Crossveinless-c, the Drosophila homolog of tumor suppressor DLC1, regulates directional elongation of dendritic branches via down-regulating Rho1 activity. Genes Cells 15(5): 485--500. (Export to RIS)
FlyBase ID	FBrf0211000
Publication Type	Research paper
PubMed ID	20384791
PubMed Abstract	Diverse neuronal subtypes develop distinctive morphologies of dendritic arbors that receive synaptic or sensory inputs. Dendritic arbors of many subtypes take on a polarized shape, and one underlying mechanism is unidirectionally biased elongation of dendritic branches. As reported herein, we found that Drosophila Crossveinless-c (Cv-c) was a key regulator for such directional growth. In the cv-c mutant, two subclass of multidendritic sensory neurons examined formed dorsally directed branches; however, dendritic branches had difficulty in growing along the anterior-posterior (A-P) body axis. Cv-c belongs to the family of Rho GTPase-activating proteins (RhoGAPs) and is the homolog of human tumor suppressor DLC1. The RhoGAP activity of Cv-c was required cell-autonomously for the A-P-oriented growth, and Cv-c elevated the GTPase activity of Rho1 and Cdc42 in a cell-free assay. Our analysis of genetic interactions suggested that Rho1 was the target of Cv-c in vivo. All of our results suggest that Cv-c contributes to sprouting and subsequent growth of the A-P-oriented branches through negative regulation of Rho1. We discuss a role of Cv-c in dendritic growth in response to environmental cues.
DOI	10.1111/j.1365-2443.2010.01399.x
Related Publication(s)
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Associated Information
Comments
Associated Files
Other Information
Secondary IDs
Language of Publication	English
Additional Languages of Abstract
Also Published As
Parent Publication
Publication Type	Journal
Abbreviation	Genes Cells
Title	Genes to cells : devoted to molecular & cellular mechanisms
Publication Year	1996-
ISBN/ISSN	1356-9597
Data from Reference
Alleles (26)
	18w^Scer\UAS.cRa Avic\GFP^{S65T.Scer\UAS} Avic\GFP^{Venus.Scer\UAS.T:Hsap\pm} Cdc42^N17.Scer\UAS cv-c¹²⁶⁰ cv-c⁰⁶⁹⁵¹ cv-c^f04940 cv-c^f07633 cv-c^{L.Scer\UAS.T:Avic\GFP-EGFP} cv-c^L.Scer\UAS cv-c^{R601L.S.Scer\UAS} cv-c^{S.Scer\UAS.T:Avic\GFP-EGFP} cv-c^S.Scer\UAS cv-c^{ΔC.S.Scer\UAS} cv-c^{ΔN.S.Scer\UAS} cv-c^{ΔS.S.Scer\UAS} Mmus\Cd8a^{Scer\UAS.T:Avic\GFP} Rac1^N17.Scer\UAS Rho1^N19.Scer\UAS Scer\GAL4^{CG33523-NP4600} Scer\GAL4^da.G32 Scer\GAL4^IG1-1 Scer\GAL4^NP1015 Scer\GAL4^NP2225 T:Avic\GFP^EGFP w^+mC
Constructs (16)
	P{GAL4-da.G32} P{UAS-18w.R} P{UAS-Cdc42.N17} P{UAS-cv-c.L.EGFP} P{UAS-cv-c.L} P{UAS-cv-c.S.EGFP} P{UAS-cv-c.S.R601L} P{UAS-cv-c.S.ΔC} P{UAS-cv-c.S.ΔN} P{UAS-cv-c.S.ΔS} P{UAS-cv-c.S} P{UAS-GFP.S65T} P{UAS-mCD8::GFP.L} P{UAS-Rac1.N17} P{UAS-Rho1.N19} P{UAS-Venus.pm}
Genes (10)
	18w Avic\GFP Cdc42 cv-c futsch Mmus\Cd8a Rac1 Rho1 Scer\GAL4 w
Insertions (4)
	P{GawB}ab^NP2225 P{GawB}CG33523^NP4600 P{GawB}IG1-1 P{GawB}Rip11^NP1015
Natural transposons (1)
	P-element