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| Citation | Gan, Q., Schones, D.E., Ho Eun, S., Wei, G., Cui, K., Zhao, K., Chen, X. (2010). Monovalent and unpoised status of most genes in undifferentiated cell-enriched Drosophila testis. Genome Biol. 11(4): R42. (Export to RIS) | ||
| FlyBase ID | FBrf0211027 | ||
| Publication Type | Research paper | ||
| PubMed ID | 20398323 | ||
| PubMed Abstract | Increasing evidence demonstrates that stem cells maintain their identities by a unique transcription network and chromatin structure. Opposing epigenetic modifications H3K27 me3 and H3K4 me3 have been proposed to label differentiation-associated genes in stem cells, progenitor and precursor cells. In addition, many differentiation-associated genes are maintained at a poised status by recruitment of the initiative RNA Polymerase II (Pol II) at their promoter regions, in preparation for lineage-specific expression upon differentiation. Previous studies have been performed using cultured mammalian embryonic stem cells. To a lesser extent, chromatin structure has been delineated in other model organisms, such as Drosophila, to open new avenues for genetic analyses.Here we use testes isolated from a Drosophila bag of marbles mutant strain, from which germ cells are in their undifferentiated status. We use these testes to study the endogenous chromatin structure of undifferentiated cells using ChIP-seq. We integrate the ChIP-seq with RNA-seq data, which measures the digital transcriptome. Our genome-wide analyses indicate that most differentiation-associated genes in undifferentiated cells lack an active chromatin mark and initiative Pol II; instead, they are associated with either the repressive H3K27 me3 mark or no detectable mark.Our results reveal that most of the differentiation-associated genes in undifferentiated-cell-enriched Drosophila testes are associated with monovalent but not bivalent modifications, a chromatin signature that is distinct from the data reported in mammalian stem or precursor cells, which may reflect cell type specificity, species specificity, or both. | ||
| DOI | 10.1186/gb-2010-11-4-r42 | ||
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| Language of Publication | English | ||
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| Publication Type | Journal | ||
| Abbreviation | Genome Biol. | ||
| Title | Genome Biology | ||
| Publication Year | 2000- | ||
| ISBN/ISSN | 1465-6906 | ||
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Data from Reference
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| Genes (5)
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