Reference Report

Reference
Citation	Jones, W.M., Chao, A.T., Zavortink, M., Saint, R., Bejsovec, A. (2010). Cytokinesis proteins Tum and Pav have a nuclear role in Wnt regulation. J. Cell Sci. 123(13): 2179--2189. (Export to RIS)
FlyBase ID	FBrf0211056
Publication Type	Research paper
PubMed ID	20516152
PubMed Abstract	Wg/Wnt signals specify cell fates in both invertebrate and vertebrate embryos and maintain stem-cell populations in many adult tissues. Deregulation of the Wnt pathway can transform cells to a proliferative fate, leading to cancer. We have discovered that two Drosophila proteins that are crucial for cytokinesis have a second, largely independent, role in restricting activity of the Wnt pathway. The fly homolog of RacGAP1, Tumbleweed (Tum)/RacGAP50C, and its binding partner, the kinesin-like protein Pavarotti (Pav), negatively regulate Wnt activity in fly embryos and in cultured mammalian cells. Unlike many known regulators of the Wnt pathway, these molecules do not affect stabilization of Arm/beta-catenin (betacat), the principal effector molecule in Wnt signal transduction. Rather, they appear to act downstream of betacat stabilization to control target-gene transcription. Both Tum and Pav accumulate in the nuclei of interphase cells, a location that is spatially distinct from their cleavage-furrow localization during cytokinesis. We show that this nuclear localization is essential for their role in Wnt regulation. Thus, we have identified two modulators of the Wnt pathway that have shared functions in cell division, which hints at a possible link between cytokinesis and Wnt activity during tumorigenesis.
DOI	10.1242/jcs.067868
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Language of Publication	English
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Publication Type	Journal
Abbreviation	J. Cell Sci.
Title	Journal of Cell Science
Publication Year	1966-
ISBN/ISSN	0021-9533
Data from Reference
Aberrations (1)
	Df(3L)GN24
Alleles (23)
	arm⁴ arm⁸ arm^{S10.Scer\UAS.T:Hsap\MYC} Avic\GFP^Scer\UAS.cUa Ecol\lacZ^rho-X81 Mtl^Δ nkd^Scer\UAS.cZa pan^ΔN.Scer\UAS pav^B200 pav^{NLS4-7*.Scer\UAS.P\T.T:Avic\GFP-m6} pav^{Scer\UAS.P\T.T:Avic\GFP-m6} Rac1^J10 Rac2^Δ Scer\GAL4^ap-md544 Scer\GAL4^arm.PS Scer\GAL4^e22c Scer\GAL4^prd.RG1 tum^AR2 tum^Scer\UAS.cSa tum^{ΔE1E.Scer\UAS} tum^{ΔNLS.Scer\UAS} tum^{ΔPav.Scer\UAS} wg^l-17
Constructs (12)
	P{GAL4-arm.S} P{GAL4-prd.F} P{UAS-arm.S10} P{UAS-GFP.U} P{UAS-nkd.Z} P{UAS-pan.dTCFΔN} P{UASp-GFP-pav.NLS4-7*} P{UASp-GFP-pav} P{UAS-RacGAP.S} P{UAS-RacGAP.ΔE1E} P{UAS-tum.ΔNLS} P{UAS-tum.ΔPav}
Genes (16)
	arm Avic\GFP Axn Ecol\lacZ en Mtl nkd pan pav Rac1 Rac2 rho Scer\GAL4 scra tum wg
Insertions (3)
	P{en2.4-GAL4}e22c P{GawB}ap^md544 P{lwB}X81
Natural transposons (1)
	P-element