Reference Report

Reference
Citation	Coleman, H.A., Labrador, J.P., Chance, R.K., Bashaw, G.J. (2010). The Adam family metalloprotease Kuzbanian regulates the cleavage of the roundabout receptor to control axon repulsion at the midline. Development 137(14): 2417--2426. (Export to RIS)
FlyBase ID	FBrf0211121
Publication Type	Research paper
PubMed ID	20570941
PubMed Abstract	Slits and their Roundabout (Robo) receptors mediate repulsive axon guidance at the Drosophila ventral midline and in the vertebrate spinal cord. Slit is cleaved to produce fragments with distinct signaling properties. In a screen for genes involved in Slit-Robo repulsion, we have identified the Adam family metalloprotease Kuzbanian (Kuz). Kuz does not regulate midline repulsion through cleavage of Slit, nor is Slit cleavage essential for repulsion. Instead, Kuz acts in neurons to regulate repulsion and Kuz can cleave the Robo extracellular domain in Drosophila cells. Genetic rescue experiments using an uncleavable form of Robo show that this receptor does not maintain normal repellent activity. Finally, Kuz activity is required for Robo to recruit its downstream signaling partner, Son of sevenless (Sos). These observations support the model that Kuz-directed cleavage is important for Robo receptor activation.
DOI	10.1242/dev.047993
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Development
Title	Development
Publication Year	1987-
ISBN/ISSN	0950-1991
Data from Reference
Alleles (26)
	Avic\GFP^{Scer\UAS.T:Btau\MAPT,T:Hsap\MYC} comm^Δe39 fra::robo^{Scer\UAS.FR.T:Hsap\MYC} fra::robo^{U.Scer\UAS.T:Hsap\MYC} kuz¹¹² kuz⁴²⁰ kuz²⁵⁸³ kuz^DN.Scer\UAS kuz^e29-4 kuz^H143 kuz^Scer\UAS.cCa kuz^{Scer\UAS.T:Ivir\HA1} kuz^unspecified robo¹ robo⁵ robo^{Scer\UAS.T:Hsap\MYC} robo^unspecified Scer\GAL4^ap-md544 Scer\GAL4^eg-Mz360 Scer\GAL4^elav.PLu Scer\GAL4^sli.PS sli¹ sli² sli^Scer\UAS.cUa sli^{Uncleave.Scer\UAS} sli^unspecified
Constructs (11)
	P{GAL4-elav.L} P{GAL4-sli.S} P{UAS-btau::GFP.T:Myc} P{UAS-fra::robo.U} P{UAS-fra-robo-Myc} P{UAS-kuz.C} P{UAS-kuz.DN} P{UAS-kuz.HA} P{UAS-robo.Myc} P{UAS-sli.U} P{UAS-sli.Uncleave}
Genes (12)
	Avic\GFP comm Fas2 fra fra::robo kuz robo Scer\GAL4 sli Sos T:Hsap\MYC T:Ivir\HA1
Insertions (2)
	P{GawB}ap^md544 P{GawB}eg^Mz360
Natural transposons (1)
	P-element