Reference Report

Reference
Citation	Walrad, P.B., Hang, S., Joseph, G.S., Salas, J., Gergen, J.P. (2010). Distinct contributions of conserved modules to runt transcription factor activity. Mol. Biol. Cell 21(13): 2315--2326. (Export to RIS)
FlyBase ID	FBrf0211193
Publication Type	Research paper
PubMed ID	20462957
PubMed Abstract	Runx proteins play vital roles in regulating transcription in numerous developmental pathways throughout the animal kingdom. Two Runx protein hallmarks are the DNA-binding Runt domain and a C-terminal VWRPY motif that mediates interaction with TLE/Gro corepressor proteins. A phylogenetic analysis of Runt, the founding Runx family member, identifies four distinct regions C-terminal to the Runt domain that are conserved in Drosophila and other insects. We used a series of previously described ectopic expression assays to investigate the functions of these different conserved regions in regulating gene expression during embryogenesis and in controlling axonal projections in the developing eye. The results indicate each conserved region is required for a different subset of activities and identify distinct regions that participate in the transcriptional activation and repression of the segmentation gene sloppy-paired-1 (slp1). Interestingly, the C-terminal VWRPY-containing region is not required for repression but instead plays a role in slp1 activation. Genetic experiments indicating that Groucho (Gro) does not participate in slp1 regulation further suggest that Runt's conserved C-terminus interacts with other factors to promote transcriptional activation. These results provide a foundation for further studies on the molecular interactions that contribute to the context-dependent properties of Runx proteins as developmental regulators.
DOI	10.1091/mbc.E09-11-0953
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Mol. Biol. Cell
Title	Molecular Biology of the Cell
Publication Year	1992-
ISBN/ISSN	1059-1524
Data from Reference
Aberrations (1)
	Df(3R)Espl22
Alleles (14)
	ftz^Scer\UAS.cLa gro^E48 opa^Scer\UAS.cSa Rpd3⁰⁴⁵⁵⁶ run^CK.Scer\UAS run^Scer\UAS.cLa run^{Scer\UAS.T:Zzzz\FLAG} run^{Δ3.Scer\UAS.T:Zzzz\FLAG} run^{Δ6.Scer\UAS.T:Zzzz\FLAG} run^{Δ7.Scer\UAS.T:Zzzz\FLAG} run^{Δ8.Scer\UAS.T:Zzzz\FLAG} Scer\GAL4^MT14 Scer\GAL4^nos.PG Scer\GAL4^Sgs3.PD
Constructs (11)
	P{GAL4-nos.NGT} P{Sgs3-GAL4.PD} P{UAS-ftz.L} P{UAS-opa.VZ} P{UAS-run.CK} P{UAS-run.L} P{UAS-runt.FLAG} P{UAS-runt.Δ3} P{UAS-runt.Δ6} P{UAS-runt.Δ7} P{UAS-runt.Δ8}
Genes (15)
	Dere\run Dper\run Dpse\run Dsim\run Dvir\run Dyak\run en ftz gro opa Rpd3 run Scer\GAL4 slp1 T:Zzzz\FLAG
Insertions (1)
	P{GawB}MT14
Natural transposons (1)
	P-element