Reference Report

Reference
Citation	Brown, J.L., Kassis, J.A. (2010). Spps, a Drosophila Sp1/KLF family member, binds to PREs and is required for PRE activity late in development. Development 137(15): 2597--2602. (Export to RIS)
FlyBase ID	FBrf0211271
Publication Type	Research paper
PubMed ID	20627963
PubMed Abstract	The Polycomb group of proteins (PcG) is important for transcriptional repression and silencing in all higher eukaryotes. In Drosophila, PcG proteins are recruited to the DNA by Polycomb-group response elements (PREs), regulatory sequences whose activity depends on the binding of many different sequence-specific DNA-binding proteins. We previously showed that a binding site for the Sp1/KLF family of zinc-finger proteins is required for PRE activity. Here, we report that the Sp1/KLF family member Spps binds specifically to Ubx and engrailed PREs, and that Spps binds to polytene chromosomes in a pattern virtually identical to that of the PcG protein, Psc. A deletion of the Spps gene causes lethality late in development and a loss in pairing-sensitive silencing, an activity associated with PREs. Finally, the Spps mutation enhances the phenotype of pho mutants. We suggest that Spps may work with, or in parallel to, Pho to recruit PcG protein complexes to PREs.
DOI	10.1242/dev.047761
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Secondary IDs
Language of Publication	English
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Also Published As
Parent Publication
Publication Type	Journal
Abbreviation	Development
Title	Development
Publication Year	1987-
ISBN/ISSN	0950-1991
Data from Reference
Aberrations (2)
	Df(3R)crb87-4 Df(3R)Exel6198
Alleles (8)
	Pc⁴ pho¹ Psc¹ Scer\GAL4^en-e16E Spps¹ w^en.8 w^en.PRE w^Ubx.PRE.D
Constructs (3)
	P{en-w.PRE} P{Ubx-w.PRE.D} P{w^en.8}
Genes (6)
	Pc pho Psc Scer\GAL4 Spps w
Insertions (5)
	P{en2.4-GAL4}e16E P{en-w.PRE}4-8-3-1B P{Mae-UAS.6.11}Spps^DP01353 P{Ubx-w.PRE.D}K2-1 P{w^en.8}10C
Natural transposons (1)
	P-element