Reference Report

Reference
Citation	Kaplan, Y., Gibbs-Bar, L., Kalifa, Y., Feinstein-Rotkopf, Y., Arama, E. (2010). Gradients of a Ubiquitin E3 Ligase Inhibitor and a Caspase Inhibitor Determine Differentiation or Death in Spermatids. Dev. Cell 19(1): 160--173. (Export to RIS)
FlyBase ID	FBrf0211287
Publication Type	Research paper
PubMed ID	20643358
PubMed Abstract	Caspases are executioners of apoptosis but also participate in a variety of vital cellular processes. Here, we identified Soti, an inhibitor of the Cullin-3-based E3 ubiquitin ligase complex required for caspase activation during Drosophila spermatid terminal differentiation (individualization). We further provide evidence that the giant inhibitor of apoptosis-like protein dBruce is a target for the Cullin-3-based complex, and that Soti competes with dBruce for binding to Klhl10, the E3 substrate recruitment subunit. We then demonstrate that Soti is expressed in a subcellular gradient within spermatids and in turn promotes proper formation of a similar dBruce gradient. Consequently, caspase activation occurs in an inverse graded fashion, such that the regions of the developing spermatid that are the last to individualize experience the lowest levels of activated caspases. These findings elucidate how the spatial regulation of caspase activation can permit caspase-dependent differentiation while preventing full-blown apoptosis.
DOI	10.1016/j.devcel.2010.06.009
Related Publication(s)
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Associated Information
Comments
Associated Files
Other Information
Secondary IDs
Language of Publication	English
Additional Languages of Abstract
Also Published As
Parent Publication
Publication Type	Journal
Abbreviation	Dev. Cell
Title	Developmental Cell
Publication Year	2001-
ISBN/ISSN
Data from Reference
Aberrations (1)
	Df(3R)Exel6267
Alleles (17)
	Avic\GFP^Scer\UAS.cUa Cul-3⁰⁶⁴³⁰ Cul-3^gft2 Cul-3^mds1 Cul-3^mds5 jar¹ klhl10³ klhl10⁴ klhl10^{G496R.Scer\UAS} klhl10^Scer\UAS.cKa Scer\GAL4^GMR.PU soti^3KR.soti soti^{3KR.T:Hsap\MYC} soti^+ttr soti^Scer\UAS.cKa soti^sik soti^T:Hsap\MYC
Constructs (7)
	P{GMR-GAL4.U} P{tr-soti.3KR} P{tr-soti.MYC} P{tr-soti} P{UAS-GFP.U} P{UAS-klhl10.G496R} P{UAS-klhl10.K}
Genes (10)
	Avic\GFP Bruce Cul-3 jar klhl10 Roc1a rpr Scer\GAL4 soti T:Hsap\MYC
Insertions (3)
	P{PZ}Cul-3⁰⁶⁴³⁰ P{PZ}jar¹ P{XP}d01837
Natural transposons (1)
	P-element