Reference Report

Reference
Citation	Cooper, M.T., Conant, A.W., Kennison, J.A. (2010). Molecular Genetic Analysis of Chd3 and Polytene Chromosome Region 76B-D in Drosophila melanogaster. Genetics 185(3): 811--822. (Export to RIS)
FlyBase ID	FBrf0211329
Publication Type	Research paper
PubMed ID	20439780
PubMed Abstract	The Drosophila melanogaster Chd3 gene encodes a member of the CHD group of SNF2/RAD54 ATPases. CHD proteins are conserved from yeast to man and many are subunits of chromatin-remodeling complexes that facilitate transcription. Drosophila CHD3 proteins are not found in protein complexes, but as monomers that remodel chromatin in vitro. CHD3 colocalize with elongating RNA polymerase II on salivary gland polytene chromosomes. Since the role of Chd3 in development was unknown, we isolated and characterized the essential genes within the 640-kb region of the third chromosome (polytene chromosome region 76B-D) that includes Chd3. We recovered mutations in 24 genes that are essential for zygotic viability. We found that transposon-insertion mutants for 46% of the essential genes are included in the Drosophila Gene Disruption Project collection. None of the essential genes that we identified are in a 200-kb region that includes Chd3. We generated a deletion of Chd3 by targeted gene replacement. This deletion had no effect on either viability or fertility.
DOI	10.1534/genetics.110.115121
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Genetics
Title	Genetics
Publication Year	1916-
ISBN/ISSN	0016-6731
Data from Reference
Aberrations (27)
	Df(3L)25-21 Df(3L)A23 Df(3L)ash1^A16 Df(3L)BSC2 Df(3L)BSC20 Df(3L)BSC417 Df(3L)BSC445 Df(3L)ED228 Df(3L)ED4799 Df(3L)ED4858 Df(3L)Exel6135 Df(3L)Exel9007 Df(3L)Exel9008 Df(3L)Exel9009 Df(3L)Exel9011 Df(3L)Exel9045 Df(3L)Exel9046 Df(3L)kto2 Df(3L)kto22 Df(3L)pipe^A13 Df(3L)XS411 Df(3L)XS533 Df(3L)XS543 Df(3L)XS572 Df(3L)XS705 Df(3L)XS917 Df(3L)XS2182
Alleles (31)
	ash1⁶ ash1^MB03235 Chd3¹ Gyc76C^A377 Gyc76C^KG02500 HLH106¹ HLH106² HLH106³ kto¹ l(3)76BDm^G14537 l(3)76BDm^L3809 l(3)76BDr^DG04109 Mi-2⁰¹⁰⁵⁸ Mi-2^DG14402 Mi-2^EY08696 Mi-2^j3D4 Mi-2^L1243 Mi-2^unspecified Papss^B412 Papss^EY08999 pip^C14 Rab8^B229 serp^e02821 Shal^f00495 Su(Tpl)^DG12505 Su(Tpl)^EY08138 Su(Tpl)^j3D4 Su(Tpl)^unspecified Taf6^f06930 trpml¹ verm^KG07819
Constructs (1)
	P{EPg}
Genes (99)
	825-Oak asf1 ash1 brv1 CG7770 CG8004 CG8334 CG8765 CG8786 CG9231 CG9279 CG9330 CG9368 CG9372 CG9376 CG9392 CG9449 CG9451 CG9452 CG10445 CG12519 CG13051 CG13066 CG13067 CG13069 CG13674 CG13678 CG13679 CG14087 CG14095 CG14096 CG14100 CG14101 CG14102 CG14103 CG14104 CG15881 CG18294 CG32206 CG32212 CG32213 CG32214 CG32221 CG33062 CG33255 CG34116 CG42529 CG42637 CG42638 Chd3 Cpr76Ba Cpr76Bb Cpr76Bc Cpr76Bd CR32205 CR32207 cyc Cyp305a1 Deaf1 Fibp Grasp65 Gyc76C Gβ76C HLH106 Ir76a Kap-α1 kto l(3)76BDd l(3)76BDg l(3)76BDh l(3)76BDj l(3)76BDm l(3)76BDr l(3)76BDt lds Lon lush Mi-2 mir-9a mir-33 Mtr3 Oat Papss pip pncr009:3L Prp3 Rab8 RhoGDI Rpn1 serp Shal Su(Tpl) Su(z)12 Taf6 tey trc trpml verm wnd
Insertions (24)
	Mi{ET1}ash1^MB03235 P{EP}l(3)76BDm^G14537 P{EPg}HP30622 P{EPgy2}EY03157 P{EPgy2}Mi-2^EY08696 P{EPgy2}Papss^EY08999 P{EPgy2}Su(Tpl)^EY08138 P{lacW}l(3)76BDm^L3809 P{lacW}Mi-2^j3D4 P{lacW}Mi-2^L1243 P{lacW}trc^S066917a P{PZ}Mi-2⁰¹⁰⁵⁸ P{SUPor-P}Gyc76C^KG02500 P{SUPor-P}verm^KG07819 P{wHy}l(3)76BDr^DG04109 P{wHy}Mi-2^DG14402 P{wHy}Su(Tpl)^DG12505 PBac{3HPy⁺}C260 PBac{5HPw⁺}Gyc76C^A377 PBac{5HPw⁺}Papss^B412 PBac{5HPw⁺}Rab8^B229 PBac{RB}serp^e02821 PBac{WH}Shal^f00495 PBac{WH}Taf6^f06930
Natural transposons (1)
	Doc