Reference Report

Reference
Citation	Hermle, T., Saltukoglu, D., Grünewald, J., Walz, G., Simons, M. (2010). Regulation of Frizzled-Dependent Planar Polarity Signaling by a V-ATPase Subunit. Curr. Biol. 20(14): 1269--1276. (Export to RIS)
FlyBase ID	FBrf0211335
Publication Type	Research paper
PubMed ID	20579879
PubMed Abstract	Frizzled (Fz) is a seven-pass transmembrane receptor that acts in both Wingless (Wg) and planar cell polarity (PCP) pathways. A prerequisite for PCP signaling is the asymmetric subcellular distribution of Fz. However, the regulation of Fz asymmetry is currently not well understood. Here we describe that the transmembrane protein CG8444 (here termed VhaPRR) is needed for PCP signaling in Drosophila. VhaPRR is an accessory subunit of the vacuolar (V)-ATPase proton pump, but it also functions as a receptor for (pro)renin (PRR) in mammals. We show that VhaPRR function is tightly linked with Fz but not other PCP core proteins. Fz fails to localize asymmetrically in the absence of VhaPRR, and this is accompanied by prehair mispolarization of pupal wing cells. In addition, VhaPRR forms a protein complex with Fz receptors and interacts genetically with Fz in the Drosophila eye. VhaPRR also acts as a modulator of canonical Wnt signaling in larval and adult wing tissue. Its loss leads to an expansion of the Wg morphogen gradient and a reduction of Wg target gene expression. The requirement for additional V-ATPase subunits suggests that proton fluxes contribute to normal Fz receptor function and signaling.
DOI	10.1016/j.cub.2010.05.057
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Curr. Biol.
Title	Current Biology
Publication Year	1991-
ISBN/ISSN	0960-9822
Data from Reference
Alleles (16)
	dsh^Scer\UAS.cNa fz^Scer\UAS.cAa Hsap\ATP6AP2^{Scer\UAS.T:Avic\GFP-EGFP} Scer\GAL4^ap-md544 Scer\GAL4^bbg-C96 Scer\GAL4^dpp.blk1 Scer\GAL4^en-e16E Scer\GAL4^hs.2sev Scer\GAL4^nub-AC-62 stan^Scer\UAS.cUa T:Disc\RFP^mCherry VhaM8.9^EY03616 VhaM8.9^EY03616-ex VhaM8.9^GD2871 VhaM8.9^KK107676 VhaM8.9^{Scer\UAS.T:Disc\RFP-mCherry}
Constructs (9)
	P{GAL4-dpp.blk1} P{GAL4-Hsp70.sev} P{GD2871} P{KK107676} P{UAS-dsh.N} P{UAS-fmi.U} P{UAS-fz.A} P{UAS-hPRR.EGFP} P{UAS-PRR.mCherry}
Genes (13)
	Dll dlp dsh fz fz2 Hsap\ATP6AP2 Scer\GAL4 sens shg stan Vha55 VhaM8.9 wg
Insertions (5)
	P{en2.4-GAL4}e16E P{EPgy2}VhaM8.9^EY03616 P{GawB}ap^md544 P{GawB}bbg^C96 P{GawB}nubbin-AC-62
Natural transposons (1)
	P-element