Reference Report

Reference
Citation	Blanco, J., Pauli, T., Seimiya, M., Udolph, G., Gehring, W.J. (2010). Genetic interactions of eyes absent, twin of eyeless and orthodenticle regulate sine oculis expression during ocellar development in Drosophila. Dev. Biol. 344(2): 1088--1099. (Export to RIS)
FlyBase ID	FBrf0211396
Publication Type	Research paper
PubMed ID	20580700
PubMed Abstract	The homeobox gene sine oculis (so) is required for the development of the entire visual system in Drosophila, which includes the compound eyes, the ocelli, the optic lobe of the brain and the Bolwig's organ. During ocelli development, so expression labels, together with eyes absent (eya), the emergence of the ocellar precursor cells in the third instar eye-antennal disc. Footprinting and misexpression studies have led to the proposal that the Pax6 homologue twin of eyeless (toy) directly regulates the initiation of so expression in ocellar precursor cells. However, so expression in a toy loss-of-function mutant background has not been yet analyzed due to the lack of eye-antennal disc development in strong toy mutant alleles. Using an embryonic eye primordium-specific enhancer of toy, we have rescued the developmental defect of a strong toy mutant allele and analyzed so expression in the ocelli primordium of toy loss-of-function eye-antennal discs during third instar larva. The results show that so expression is only marginally affected in the absence of Toy transcriptional activity and that the toy positive effect on so expression is largely eya-mediated. These results suggest that eya is the main factor controlling both initiation and maintenance of so expression in ocellar precursor cells. In addition, we present the characterization of a new minimal eye/ocellus-specific enhancer of the so gene.
DOI	10.1016/j.ydbio.2010.05.494
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Dev. Biol.
Title	Developmental Biology
Publication Year	1959-
ISBN/ISSN	0012-1606
Data from Reference
Alleles (24)
	Ecol\lacZ^so.EEP Ecol\lacZ^toy.0.6 Ecol\lacZ^toy.0.9 Ecol\lacZ^toy.1 Ecol\lacZ^toy.2 Ecol\lacZ^toy.4.6 Ecol\lacZ^toy.8 ey^Scer\UAS.cHa eya^cli-IID eya^Scer\UAS.cBa Mmus\Cd8a^{Scer\UAS.T:Avic\GFP} oc^otd-YH13 oc^Scer\UAS.cBa oc^γa1 Scer\GAL4^dpp.blk1 Scer\GAL4^so.7 Scer\GAL4^toy.EEP Scer\GAL4^αTub84B.PL Scer\GAL80^αTub84B.PL so¹ so³ so^so11-soAE toy^hdl toy^Scer\UAS.cCa
Constructs (18)
	P{GAL4-dpp.blk1} P{so7-GAL4} P{so11-soAE-so} P{toy-GAL4.EEP} P{toy-lacZ.0.6} P{toy-lacZ.0.9} P{toy-lacZ.1} P{toy-lacZ.2} P{toy-lacZ.4.6} P{toy-lacZ.8} P{toy-lacZ.EEP} P{tubP-GAL4} P{tubP-GAL80} P{UAS-ey.H} P{UAS-eya.B.I} P{UAS-mCD8::GFP.L} P{UAS-otd.B} P{UAS-toy.C}
Genes (10)
	Ecol\lacZ ey eya eyg Mmus\Cd8a oc Scer\GAL4 Scer\GAL80 so toy
Natural transposons (1)
	P-element
Sequence features (6)
	so_so11 so_so11+soAE so_so11delTOY+soAE toy_EEP toy_fragment1 toy_fragment2