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Reference Report
| Reference | |||
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| Citation | Kuromi, H., Ueno, K., Kidokoro, Y. (2010). Two types of Ca channel linked to two endocytic pathways coordinately maintain synaptic transmission at the Drosophila synapse. Europ. J. Neurosci. 32(3): 335--346. (Export to RIS) | ||
| FlyBase ID | FBrf0211498 | ||
| Publication Type | Research paper | ||
| PubMed ID | 20704589 | ||
| PubMed Abstract | Endocytosis at the presynaptic terminal is initiated by Ca(2+) influx through voltage-gated Ca(2+) channels. At the Drosophila neuromuscular junction, we demonstrated two components of endocytosis linked to distinct Ca(2+) channels. A voltage-gated Ca(2+) channel blocker, (R)-(+)-Bay K8644 (R-BayK), selectively blocked one component (R-BayK-sensitive component) without affecting exocytosis, while low concentrations of La(3+) preferentially depressed the other component (La(3+) -sensitive component). In a temperature-sensitive mutant, shibire(ts), at non-permissive temperatures, dynamin clusters were found immunohistochemically at the active zone (AZ) during the R-BayK-sensitive endocytosis, while they were detected at the non-AZ during the La(3+)-sensitive endocytosis. Immunostaining of the Ca(2+) channel alpha(2)delta subunit encoded by straightjacket (stj) was found within the AZ, and a mutation in stj depressed the R-BayK-sensitive component but enhanced the La(3+) -sensitive one, indicating that the alpha(2)delta subunit is associated with the R-BayK-sensitive Ca(2+) channel. Filipin bound to the non-AZ membrane and inhibited the La(3+) -sensitive component, but not the R-BayK-sensitive one. We concluded that the R-BayK-sensitive component of endocytosis occurred at the AZ and termed this AZ endocytosis. We also concluded that the La(3+) -sensitive component occurred at the non-AZ and termed this non-AZ endocytosis. These two types of endocytosis were modulated by various drugs towards opposite directions, indicating that they were differentially regulated. During high-frequency stimulation, AZ endocytosis operated mainly in the early phase, whereas non-AZ endocytosis operated in the late phase. Thus, intense synaptic transmission is coordinately maintained by synaptic vesicle recycling initiated by Ca(2+) influx through the two types of Ca(2+) channel. | ||
| DOI | 10.1111/j.1460-9568.2010.07300.x | ||
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| Language of Publication | English | ||
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| Publication Type | Journal | ||
| Abbreviation | Europ. J. Neurosci. | ||
| Title | European Journal of Neuroscience | ||
| Publication Year | 1989- | ||
| ISBN/ISSN | 0953-816X | ||
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Data from Reference
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| Alleles (15)
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| Constructs (2)
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| Genes (12)
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| Insertions (8)
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