Reference Report

Reference
Citation	Mazock, G.H., Das, A., Base, C., Dubreuil, R.R. (2010). Transgene Rescue Identifies an Essential Function for Drosophila {beta} Spectrin in the Nervous System and a Selective Requirement for Ankyrin-2-binding Activity. Mol. Biol. Cell 21(16): 2860--2868. (Export to RIS)
FlyBase ID	FBrf0211527
Publication Type	Research paper
PubMed ID	20573981
PubMed Abstract	The protein spectrin is ubiquitous in animal cells and is believed to play important roles in cell shape and membrane stability, cell polarity, and endomembrane traffic. Experiments here were undertaken to identify sites of essential beta spectrin function in Drosophila and to determine whether spectrin and ankyrin function are strictly linked to one another. The Gal4-UAS system was used to drive tissue-specific overexpression of a beta spectrin transgene or to knock down beta spectrin expression with dsRNA. The results show that 1) overexpression of beta spectrin in most of the cell types studied was lethal; 2) knockdown of beta spectrin in most tissues had no detectable effect on growth or viability of the organism; and 3) nervous system-specific expression of a UAS-beta spectrin transgene was sufficient to overcome the lethality of a loss-of-function beta spectrin mutation. Thus beta spectrin expression in other cells was not required for development of fertile adult males, although females lacking nonneuronal spectrin were sterile. Previous data indicated that binding of the DAnk1 isoform of ankyrin to spectrin was partially dispensable for viability. Domain swap experiments here uncovered a different requirement for neuronal DAnk2 binding to spectrin and establish that DAnk2-binding is critical for beta spectrin function in vivo.
DOI	10.1091/mbc.E10-03-0180
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Mol. Biol. Cell
Title	Molecular Biology of the Cell
Publication Year	1992-
ISBN/ISSN	1059-1524
Data from Reference
Alleles (22)
	Ank2^{S.Scer\UAS.T:Avic\GFP-EGFP} Ank^{Scer\UAS.T:Avic\GFP-EGFP} Scer\GAL4^2-21 Scer\GAL4^bab1-Agal4-5 Scer\GAL4^Bx-MS1096 Scer\GAL4^Cg.PA Scer\GAL4^elav-C155 Scer\GAL4^how-24B Scer\GAL4^hs.PB Scer\GAL4^mex1.2.1 Scer\GAL4^P2.4.Pdf Scer\GAL4^phm.PO Scer\GAL4^rn-GAL4-14 Scer\GAL4^αTub84B.PL T:Avic\GFP^EGFP α-Spec::β-Spec^{α8.Ubi-p63E.T:Hsap\MYC} α-Spec::β-Spec^{α13.Ubi-p63E.T:Hsap\MYC} α-Spec^{Scer\UAS.T:Hsap\MYC} β-Spec^{dsRNA.Scer\UAS} β-Spec^em6 β-Spec^{M.Scer\UAS.T:Hsap\MYC} β-Spec^S012
Constructs (14)
	P{Cg-GAL4.A} P{GAL4-Hsp70.PB} P{mex1-GAL4.2.1} P{Pdf-GAL4.P2.4} P{phm-GAL4.O} P{tubP-GAL4} P{UAS-Ank2.S.EGFP} P{UAS-Ank.EGFP} P{UAS-α-Spec-myc} P{UAS-β-Spec.dsRNA} P{UAS-β-Spec.Myc} P{UAS-β-Spec-myc} P{Ubi-p63E-α-Spec::β-Spec.α8} P{Ubi-p63E-α-Spec::β-Spec.α13}
Genes (7)
	Ank Ank2 Scer\GAL4 T:Hsap\MYC α-Spec α-Spec::β-Spec β-Spec
Insertions (10)
	P{GawB}2-21 P{GawB}bab1^Agal4-5 P{GawB}Bx^MS1096 P{GawB}elav^C155 P{GawB}how^24B P{GawB}rn^GAL4-14 P{UAS-α-Spec-myc}27 P{UAS-α-Spec-myc}37 P{UAS-β-Spec.Myc}62 P{UAS-β-Spec.Myc}95
Natural transposons (1)
	P-element