Reference Report

Reference
Citation	Nezis, I.P., Shravage, B.V., Sagona, A.P., Lamark, T., Bjørkøy, G., Johansen, T., Rusten, T.E., Brech, A., Baehrecke, E.H., Stenmark, H. (2010). Autophagic degradation of dBruce controls DNA fragmentation in nurse cells during late Drosophila melanogaster oogenesis. J. Cell Biol. 190(4): 523--531. (Export to RIS)
FlyBase ID	FBrf0211647
Publication Type	Research paper
PubMed ID	20713604
PubMed Abstract	Autophagy is an evolutionarily conserved pathway responsible for degradation of cytoplasmic material via the lysosome. Although autophagy has been reported to contribute to cell death, the underlying mechanisms remain largely unknown. In this study, we show that autophagy controls DNA fragmentation during late oogenesis in Drosophila melanogaster. Inhibition of autophagy by genetically removing the function of the autophagy genes atg1, atg13, and vps34 resulted in late stage egg chambers that contained persisting nurse cell nuclei without fragmented DNA and attenuation of caspase-3 cleavage. The Drosophila inhibitor of apoptosis (IAP) dBruce was found to colocalize with the autophagic marker GFP-Atg8a and accumulated in autophagy mutants. Nurse cells lacking Atg1 or Vps34 in addition to dBruce contained persisting nurse cell nuclei with fragmented DNA. This indicates that autophagic degradation of dBruce controls DNA fragmentation in nurse cells. Our results reveal autophagic degradation of an IAP as a novel mechanism of triggering cell death and thereby provide a mechanistic link between autophagy and cell death.
DOI	10.1083/jcb.201002035
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Language of Publication	English
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Publication Type	Journal
Abbreviation	J. Cell Biol.
Title	Journal of Cell Biology
Publication Year	1966-
ISBN/ISSN	0021-9525
Data from Reference
Alleles (13)
	Atg1^Δ3D Atg8a^{Scer\UAS.P\T.T:Disc\RFP-mCherry} Atg8a^{Scer\UAS.T:Avic\GFP-EGFP,T:Disc\RFP-mCherry} Atg13^Δ81 Avic\GFP^Atg5-YD0603 Avic\GFP^{Atg8a-CA07477} Bruce^E16 Bruce^E81 Bruce^e00984 Pi3K59F^Δm22 Scer\GAL4^{nos.UTR.T:Hsim\VP16} T:Avic\GFP^EGFP T:Disc\RFP^mCherry
Constructs (5)
	P{GAL4::VP16-nos.UTR} P{PTT-GA} P{UASp-GFP-mCherry-Atg8a} P{UASp-mCherry-Atg8a} PBac{HpaI-GFP.A}
Genes (11)
	Atg1 Atg5 Atg8a Atg13 Avic\GFP Bruce Iap2 Pi3K59F ref(2)P Scer\GAL4 th
Insertions (2)
	P{PTT-GA}Atg8a^CA07477 PBac{HpaI-GFP.A}Atg5^YD0603
Natural transposons (1)
	P-element