Reference Report

Reference
Citation	Vermehren-Schmaedick, A., Ainsley, J.A., Johnson, W.A., Davies, S.A., Morton, D.B. (2010). Behavioral Responses to Hypoxia in Drosophila Larvae Are Mediated by Atypical Soluble Guanylyl Cyclases. Genetics 186(1): 183--196. (Export to RIS)
FlyBase ID	FBrf0211824
Publication Type	Research paper
PubMed ID	20592263
PubMed Abstract	The three Drosophila atypical soluble guanylyl cyclases, Gyc-89Da, Gyc-89Db, and Gyc-88E, have been proposed to act as oxygen detectors mediating behavioral responses to hypoxia. Drosophila larvae mutant in any of these subunits were defective in their hypoxia escape response-a rapid cessation of feeding and withdrawal from their food. This response required cGMP and the cyclic nucleotide-gated ion channel, cng, but did not appear to be dependent on either of the cGMP-dependent protein kinases, dg1 and dg2. Specific activation of the Gyc-89Da neurons using channel rhodopsin showed that activation of these neurons was sufficient to trigger the escape behavior. The hypoxia escape response was restored by reintroducing either Gyc-89Da or Gyc-89Db into either Gyc-89Da or Gyc-89Db neurons in either mutation. This suggests that neurons that co-express both Gyc-89Da and Gyc-89Db subunits are primarily responsible for activating this behavior. These include sensory neurons that innervate the terminal sensory cones. Although the roles of Gyc-89Da and Gyc-89Db in the hypoxia escape behavior appeared to be identical, we also showed that changes in larval crawling behavior in response to either hypoxia or hyperoxia differed in their requirements for these two atypical sGCs, with responses to 15% oxygen requiring Gyc-89Da and responses to 19 and 25% requiring Gyc-89Db. For this behavior, the identity of the neurons appeared to be critical in determining the ability to respond appropriately.
DOI	10.1534/genetics.110.118166
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Secondary IDs
Language of Publication	English
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Parent Publication
Publication Type	Journal
Abbreviation	Genetics
Title	Genetics
Publication Year	1916-
ISBN/ISSN	0016-6731
Data from Reference
Aberrations (5)
	Df(2R)BSC309 Df(2R)BSC609 Df(2R)Exel6063 Df(3R)ED5664 Df(3R)Exel8163
Alleles (20)
	Btau\PDE5A^Scer\UAS.cBa CG17922^KK105029 CG42260^GD2159 CG42260^GD8671 Cng^GD13196 Cng^KK108314 cngl^GD3370 Crei\ChR2^{H134R.Scer\UAS.T:Disc\RFP-mCherry} for^{dsRNA.Scer\UAS} Gyc88E^GD11230 Gyc88E^S451F Gyc88E^Scer\UAS.cVa Gyc88E^V474M Gyc-89Da^e01821 Gyc-89Da^Scer\UAS.cVa Gyc-89Db^MB03197 Gyc-89Db^Scer\UAS.cVa Pkg21D^{dsRNA.Scer\UAS} Scer\GAL4^Gyc-89Da.1.9 Scer\GAL4^Gyc-89Db.3.4
Constructs (13)
	P{GD2159} P{GD3370} P{GD8671} P{GD11230} P{GD13196} P{KK105029} P{KK108314} P{UAS-for.RNAi} P{UAS-Gyc88E.V} P{UAS-Gyc-89Da.V} P{UAS-Gyc-89Db.V} P{UAS-H134R-ChR2} P{UAS-Pkg21D.RNAi}
Genes (12)
	Btau\PDE5A CG17922 CG42260 Cng cngl Crei\ChR2 for Gyc88E Gyc-89Da Gyc-89Db Pkg21D Scer\GAL4
Insertions (2)
	Mi{ET1}Gyc-89Db^MB03197 PBac{RB}Gyc-89Da^e01821
Natural transposons (1)
	P-element