Reference Report

Reference
Citation	Bohm, R.A., Welch, W.P., Goodnight, L.K., Cox, L.W., Henry, L.G., Gunter, T.C., Bao, H., Zhang, B. (2010). From the Cover: A genetic mosaic approach for neural circuit mapping in Drosophila. Proc. Natl. Acad. Sci. U.S.A. 107(37): 16378--16383. (Export to RIS)
FlyBase ID	FBrf0211833
Publication Type	Research paper
PubMed ID	20810922
PubMed Abstract	Transgenic manipulation of subsets of brain cells is increasingly used for studying behaviors and their underlying neural circuits. In Drosophila, the GAL4-upstream activating sequence (UAS) binary system is powerful for gene manipulation, but GAL4 expression is often too broad for fine mapping of neural circuits. Here, we describe the development of unique molecular genetic tools to restrict GAL4 expression patterns. Building on the GAL4-UAS system, our method adds two components: a collection of enhancer-trap recombinase, Flippase (ET-FLP), transgenic lines that provide inheritable, reproducible, and tissue-specific FLP and an FRT-dependent GAL80 "flip-in" construct that converts FLP expression into tissue-specific repression of GAL4 by GAL80. By including a UAS-encoded fluorescent protein, circuit morphology can be simultaneously marked while the circuit function is assessed using another UAS transgene. In a proof-of-principle analysis, we applied this ET-FLP-induced intersectional GAL80/GAL4 repression (FINGR) method to map the neural circuitry underlying fly wing inflation. The FINGR system is versatile and powerful in combination with the vast collection of GAL4 lines for neural circuit mapping as well as for clonal analysis based on the infusion of the yeast-derived FRT/FLP system of mitotic recombination into Drosophila. The strategies and tactics underlying our FINGR system are also applicable to other genetically amenable organisms in which transgenes including the GAL4, UAS, GAL80, and FLP factors can be applied.
DOI	10.1073/pnas.1004669107
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Supplementary material	Supporting Information. [FBrf0218154]
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Proc. Natl. Acad. Sci. U.S.A.
Title	Proceedings of the National Academy of Sciences of the United States of America
Publication Year	1915-
ISBN/ISSN	0027-8424
Data from Reference
Alleles (23)
	Gl^Δ.Scer\UAS Hsap\ATXN3^{tr.Q78.Scer\UAS.T:Ivir\HA1} Mmus\Cd8a^{Scer\UAS.T:Avic\GFP} Scer\FLP1⁵⁷ Scer\FLP1^173A Scer\FLP1^247A Scer\FLP1^276B Scer\FLP1^398A Scer\FLP1^420A Scer\FLP1^467A Scer\FLP1^505A Scer\FLP1^567A Scer\FLP1^757A Scer\FLP1^820A Scer\FLP1^1143A Scer\FLP1^ET-FLPx2 Scer\GAL4^Act5C.PP Scer\GAL4^Ccap.PP Scer\GAL4^GMR.PF Scer\GAL4^{Scer\FRT.Rnor\CD2.Act5C} Scer\GAL80^{Scer\FRT.αTub84B} Scer\GAL80^αTub84B.PB Scer\GAL80^αTub84B.PG
Constructs (10)
	P{Ccap-GAL4.P} P{ET-FLPx2} P{GAL4-Act5C(FRT.CD2).P} P{GAL4-ninaE.GMR} P{tubP(FRT.stop)GAL80} P{tubP(-FRT)GAL80} P{UAS-Gl^Δ} P{UAS-Hsap\MJD.tr-Q78} P{UAS-mCD8::GFP.L} P{αTub84B(FRT.GAL80)}
Genes (6)
	Gl Hsap\ATXN3 Mmus\Cd8a Scer\FLP1 Scer\GAL4 Scer\GAL80
Insertions (12)
	P{ET-FLPx2}57 P{ET-FLPx2}173A P{ET-FLPx2}247A P{ET-FLPx2}276B P{ET-FLPx2}398A P{ET-FLPx2}420A P{ET-FLPx2}467A P{ET-FLPx2}505A P{ET-FLPx2}567A P{ET-FLPx2}757A P{ET-FLPx2}820A P{ET-FLPx2}1143A
Natural transposons (1)
	P-element