Reference Report

Reference
Citation	Xiong, X., Wang, X., Ewanek, R., Bhat, P., Diantonio, A., Collins, C.A. (2010). Protein turnover of the Wallenda/DLK kinase regulates a retrograde response to axonal injury. J. Cell Biol. 191(1): 211--223. (Export to RIS)
FlyBase ID	FBrf0211939
Publication Type	Research paper
PubMed ID	20921142
PubMed Abstract	Regenerative responses to axonal injury involve changes in gene expression; however, little is known about how such changes can be induced from a distant site of injury. In this study, we describe a nerve crush assay in Drosophila melanogaster to study injury signaling and regeneration mechanisms. We find that Wallenda (Wnd), a conserved mitogen-activated protein kinase (MAPK) kinase kinase homologous to dual leucine zipper kinase, functions as an upstream mediator of a cell-autonomous injury signaling cascade that involves the c-Jun NH(2)-terminal kinase MAPK and Fos transcription factor. Wnd is physically transported in axons, and axonal transport is required for the injury signaling mechanism. Wnd is regulated by a conserved E3 ubiquitin ligase, named Highwire (Hiw) in Drosophila. Injury induces a rapid increase in Wnd protein concomitantly with a decrease in Hiw protein. In hiw mutants, injury signaling is constitutively active, and neurons initiate a faster regenerative response. Our data suggest that the regulation of Wnd protein turnover by Hiw can function as a damage surveillance mechanism for responding to axonal injury.
DOI	10.1083/jcb.201006039
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Language of Publication	English
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Publication Type	Journal
Abbreviation	J. Cell Biol.
Title	Journal of Cell Biology
Publication Year	1966-
ISBN/ISSN	0021-9525
Data from Reference
Alleles (26)
	bsk^{K53R.Scer\UAS} bsk^VDRC.cUa Ecol\lacZ^puc-E69 Gl^Δ.Scer\UAS hiw^ND8 hiw^{Scer\UAS.W.T:Avic\GFP-EGFP} hiw^ΔN Jra^Jbz.Scer\UAS kay^Fbz.Scer\UAS Khc⁸ Mmus\Cd8a^{Scer\UAS.T:Avic\GFP} Scer\GAL4^BG380 Scer\GAL4^eve.RRa Scer\GAL4^Rapgap1-OK6 Scer\GAL4^tey-5053A slpr^BS06 slpr^{Scer\UAS.P\T.cPa} sw¹ wit^HA3 wit^HA4 wnd¹ wnd² wnd³ wnd^{KD.Scer\UAS.T:Avic\GFP} wnd^Scer\UAS.cCa wnd^VDRC.cUa
Constructs (12)
	P{eve-GAL4.RRa} P{Jbz} P{UAS-bsk.K53R} P{UAS-Fra.Fbz} P{UAS-GFP-hiw} P{UAS-GFP-wnd.KD} P{UAS-Gl^Δ} P{UAS-mCD8::GFP.L} P{UASp-slpr.WT} P{UAS-wnd.C} P{VDRC.bsk.U} P{VDRC.wnd.U}
Genes (18)
	bsk Ecol\lacZ elav futsch Gl hiw Jra kay Khc Mmus\Cd8a puc Scer\GAL4 slpr sw T:Avic\GFP VGlut wit wnd
Insertions (4)
	P{A92}puc^E69 P{GAL4}BG380 P{GawB}OK6 P{GawB}tey^5053A
Natural transposons (1)
	P-element