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Reference Report
| Reference | |||
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| Citation | Gelbart, W.M., Emmert, D.B. (2010.10.13). FlyBase High Throughput Expression Pattern Data Beta Version. (Export to RIS) | ||
| FlyBase ID | FBrf0212041 | ||
| Publication Type | FlyBase analysis | ||
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| PubMed Abstract | |||
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| Related Publication(s) | |||
| FlyBase analysis | Calculation of RPKM to generate quantitative expression data: read-length values for modENCODE developmental timecourse RNA-Seq
data. Gelbart and Emmert, 2011.8.23, Calculation of RPKM to generate quantitative expression data: read-length values for modENCODE developmental timecourse RNA-Seq data. [FBrf0214760] |
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This section contains items that were added to this record for each release.
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| FB2013_03 | |||
| FB2013_02 | |||
| All updates | Click here to see a list of all updates to this record from FB2010_08 and on. | ||
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Associated Information
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| Comments |
Computing Expression Levels: In order to summarize these data at the gene level, it was necessary first to determine a single
value for the expression level of each gene at each developmental stage or tissue.
modENCODE RNA-Seq temporal expression coverage data for each developmental stage were intersected with FlyBase exons (D. melanogaster Annotation Release 5.26) to calculate a single value reflecting average coverage per kb per gene. Each gene data point was then classified into one of nine expression level bins, and the graphical and text summaries were produced from the binned values. For the FlyAtlas larval and adult tissue data, FlyAtlas provided mean coverage levels for Affy2 probe sets, and FlyBase intersected these probe sets with the exons of all FlyBase genes to associate each probe set with one or more FlyBase genes. These values were distributed into five expression level bins for further analysis. |
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| Associated Files | File date: 2010.10.13 ; File size: 8169049 ; File format: tar.gz ; File name: Gelbart.2010.10.13.tar.gz | ||
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Other Information
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| Language of Publication | English | ||
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Data from Reference
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| Libraries/Collections (3)
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