Open Close
Reference
Citation
Pfeiffer, B.D., Ngo, T.T., Hibbard, K.L., Murphy, C., Jenett, A., Truman, J.W., Rubin, G.M. (2010). Refinement of tools for targeted gene expression in Drosophila.  Genetics 186(2): 735--755.
FlyBase ID
FBrf0212052
Publication Type
Research paper
Abstract
A wide variety of biological experiments rely on the ability to express an exogenous gene in a transgenic animal at a defined level and in a spatially and temporally controlled pattern. We describe major improvements of the methods available for achieving this objective in Drosophila melanogaster. We have systematically varied core promoters, UTRs, operator sequences, and transcriptional activating domains used to direct gene expression with the GAL4, LexA, and Split GAL4 transcription factors and the GAL80 transcriptional repressor. The use of site-specific integration allowed us to make quantitative comparisons between different constructs inserted at the same genomic location. We also characterized a set of PhiC31 integration sites for their ability to support transgene expression of both drivers and responders in the nervous system. The increased strength and reliability of these optimized reagents overcome many of the previous limitations of these methods and will facilitate genetic manipulations of greater complexity and sophistication.
PubMed ID
PubMed Central ID
PMC2942869 (PMC) (EuropePMC)
Related Publication(s)
Personal communication to FlyBase
GCaMP6 constructs and insertions from Douglas Kim.
Kim, 2013.1.16, GCaMP6 constructs and insertions from Douglas Kim. [FBrf0220598]
LexAop-GCaMP6 stocks from Douglas Kim.
Kim, 2013.6.21, LexAop-GCaMP6 stocks from Douglas Kim. [FBrf0221946]
lexA Driver Collection of Rubin Laboratory at Janelia Farm.
Pfeiffer et al., 2013.10.9, lexA Driver Collection of Rubin Laboratory at Janelia Farm. [FBrf0222940]
lexA Driver Collection of Rubin Laboratory at Janelia Farm, Additional Lines.
Pfeiffer et al., 2013.11.22, lexA Driver Collection of Rubin Laboratory at Janelia Farm, Additional Lines. [FBrf0223587]
P{VGlut-GAL80.V} construct and insertion.
Vosshall, 2014.4.17, P{VGlut-GAL80.V} construct and insertion. [FBrf0224935]
Associated Information
Comments
Associated Files
Other Information
Secondary IDs
    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    Genetics
    Title
    Genetics
    Publication Year
    1916-
    ISBN/ISSN
    0016-6731
    Data From Reference
    Alleles (55)
    Genes (7)
    Sequence Features (5)
    Natural transposons (2)
    Insertions (37)
    Experimental Tools (16)
    Transgenic Constructs (61)