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Citation
Pauli, A., van Bemmel, J.G., Oliveira, R.A., Itoh, T., Shirahige, K., van Steensel, B., Nasmyth, K. (2010). A direct role for cohesin in gene regulation and ecdysone response in Drosophila salivary glands.  Curr. Biol. 20(20): 1787--1798.
FlyBase ID
FBrf0212124
Publication Type
Research paper
Abstract

Developmental abnormalities observed in Cornelia de Lange syndrome have been genetically linked to mutations in the cohesin machinery. These and other recent experimental findings have led to the suggestion that cohesin, in addition to its canonical function of mediating sister chromatid cohesion, might also be involved in regulating gene expression.We report that cleavage of cohesin's kleisin subunit in postmitotic Drosophila salivary glands induces major changes in the transcript levels of many genes. Kinetic analyses of changes in transcript levels upon cohesin cleavage reveal that a subset of genes responds to cohesin cleavage within a few hours. In addition, cohesin binds to most of these loci, suggesting that cohesin is directly regulating their expression. Among these genes are several that are regulated by the steroid hormone ecdysone. Cytological visualization of transcription at selected ecdysone-responsive genes reveals that puffing at Eip74EF ceases within an hour or two of cohesin cleavage, long before any decline in ecdysone receptor could be detected at this locus.We conclude that cohesin regulates expression of a distinct set of genes, including those mediating the ecdysone response.

Graphical Abstract
Obtained with permission from Cell Press.
PubMed ID
PubMed Central ID
PMC4763543 (PMC) (EuropePMC)
Related Publication(s)
Note

Gene regulation: the cohesin ring connects developmental highways.
Dorsett, 2010, Curr. Biol. 20(20): R886--R888 [FBrf0214201]

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Secondary IDs
    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    Curr. Biol.
    Title
    Current Biology
    Publication Year
    1991-
    ISBN/ISSN
    0960-9822
    Data From Reference