Reference Report

Reference
Citation	Zeng, X., Chauhan, C., Hou, S.X. (2010). Characterization of midgut stem cell- and enteroblast-specific Gal4 lines in drosophila. genesis 48(10): 607--611. (Export to RIS)
FlyBase ID	FBrf0212138
Publication Type	Research paper
PubMed ID	20681020
PubMed Abstract	The homeostasis of Drosophila midgut is maintained by multipotent intestinal stem cells (ISCs), each of which gives rise to a new ISC and an immature daughter cell, enteroblast (EB), after one asymmetric cell division. In Drosophila, the Gal4-UAS system is widely used to manipulate gene expression in a tissue- or cell-specific manner, but in Drosophila midgut, there are no ISC- or EB-specific Gal4 lines available. Here we report the generation and characterization of Dl-Gal4 and Su(H)GBE-Gal4 lines, which are expressed specifically in the ISCs and EBs separately. Additionally, we demonstrate that Dl-Gal4 and Su(H)GBE-Gal4 are expressed in adult midgut progenitors (AMPs) and niche peripheral cells (PCs) separately in larval midgut. These two Gal4 lines will serve as invaluable tools for navigating ISC behaviors.
DOI	10.1002/dvg.20661
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Secondary IDs
Language of Publication	English
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Parent Publication
Publication Type	Journal
Abbreviation	genesis
Title	genesis
Publication Year	2000-
ISBN/ISSN	1526-954X 1526-968X
Data from Reference
Alleles (10)
	Avic\GFP^Scer\UAS.cUa Dl⁰⁵¹⁵¹ Dl^05151-G Ecol\lacZ^{Ddc.be2.E(spl).m8} elav^C155 Mmus\Cd8a^{Scer\UAS.T:Avic\GFP} Scer\GAL4^Dl-05151-G Scer\GAL4^elav-C155 Scer\GAL4^esg-NP5130 Scer\GAL4^Su(H).GBE
Constructs (5)
	P{Ddc.E(spl)-lacZ.Gbe.m8} P{GawB} P{Su(H)GBE-GAL4} P{UAS-GFP.U} P{UAS-mCD8::GFP.L}
Genes (8)
	Avic\GFP Dl Ecol\lacZ elav esg Mmus\Cd8a pros Scer\GAL4
Insertions (4)
	P{GawB}Dl^05151-G P{GawB}elav^C155 P{GawB}NP5130 P{PZ}Dl⁰⁵¹⁵¹
Natural transposons (1)
	P-element