Reference Report

Reference
Citation	Silies, M., Klämbt, C. (2010). APC/C(Fzr/Cdh1)-dependent regulation of cell adhesion controls glial migration in the Drosophila PNS. Nat. Neurosci. 13(11): 1357--1364. (Export to RIS)
FlyBase ID	FBrf0212144
Publication Type	Research paper
PubMed ID	20890296
PubMed Abstract	Interactions between neurons and glia are a key feature during the assembly of the nervous system. During development, glial cells often follow extending axons, implying that axonal outgrowth and glial migration are precisely coordinated. We found that the anaphase-promoting complex/cyclosome (APC/C) co-activator fizzy-related/Cdh1 (Fzr/Cdh1) is involved in the non-autonomous control of peripheral glial migration in postmitotic Drosophila neurons. APC/C(Fzr/Cdh1) is a cell-cycle regulator that targets proteins that are required for G1 arrest for ubiquitination and subsequent degradation. We found that Fzr/Cdh1 function is mediated by the immunoglobulin superfamily cell adhesion molecule Fasciclin2 (Fas2). In motor neurons Fzr/Cdh1 is crucial for the establishment of a graded axonal distribution of Fas2. Axonal Fas2 interacts homophilically with a glial isoform of Fas2. Glial migration is initiated along axonal segments that have low levels of Fas2 but stalls in axonal domains with high levels of Fas2 on their surfaces. This represents a simple mechanism by which a subcellular gradient of adhesiveness can coordinate glial migration with axonal growth.
DOI	10.1038/nn.2656
Related Publication(s)
Supplementary material	Online Methods. [FBrf0212474]
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Associated Information
Comments
Associated Files
Other Information
Secondary IDs
Language of Publication	English
Additional Languages of Abstract
Also Published As
Parent Publication
Publication Type	Journal
Abbreviation	Nat. Neurosci.
Title	Nature Neuroscience
Publication Year	1998-
ISBN/ISSN	1097-6256
Data from Reference
Aberrations (2)
	Df(1)bi-D2 Df(2R)Chi^g230
Alleles (32)
	Avic\GFP^Fas2-CB03613 Avic\GFP^Fas2-GFP397 Avic\GFP^Fas2-rG272 CycA^C8LR1 CycB^Scer\UAS.cWa Ecol\lacZ^rap-G0418 Fas2^CB03613 Fas2^EB112 Fas2^{extra.Scer\UAS.T:Avic\GFP-YFP} Fas2^GFP397 Fas2^{intra.Scer\UAS.T:Avic\GFP-YFP} Fas2^{Scer\UAS.T:Avic\GFP-YFP} lmgA^EY11317 mr^{Scer\UAS.T:Avic\GFP-EGFP} Nrg^l4 Rab4^{SN.Scer\UAS.cTa} rap^8F3 rap^19C6 rap^20E1 rap^G129 rap^H089 rap^ie28 rap^M442 rap^Scer\UAS.cSa rap^{Scer\UAS.T:Hsap\MYC} Scer\GAL4^elav.PLu Scer\GAL4^elav.PU Scer\GAL4^elav-C155 Scer\GAL4^Fas2-Mz507 Scer\GAL4^gcm.PU Scer\GAL4^repo.PU shi^1.Scer\UAS
Constructs (13)
	M{UAS-mr.EGFP} P{GAL4-elav.L} P{gcm-GAL4.U} P{lacW} P{repo-GAL4.U} P{UAS-CycB.W} P{UAS-extra-Fas2.YFP} P{UAS-Fas2.YFP} P{UAS-intra-Fas2.YFP} P{UAS-myc-fzr} P{UAS-Rab4.SN.T} P{UAS-shi^ts1.K} P{unk}
Genes (18)
	Avic\GFP Cdc27 CycA CycB Ecol\lacZ Fas2 ida lmgA mr Nrg Rab4 Rab5 Rab11 rap repo Scer\GAL4 shi shtd
Insertions (6)
	P{GAL4}Mz507 P{GawB}elav^C155 P{lacW}Fas2^rG272 P{lacW}rap^G0418 P{PTT-GB}Fas2^CB03613 P{unk}Fas2^GFP397
Natural transposons (2)
	Dmau\mariner P-element
Transcripts (4)
	Avic\GFP^Fas2-GFP397RA Avic\GFP^Fas2-rG272RA Ecol\lacZ^rap-G0418RA Scer\GAL4^Fas2-Mz507RA