Reference Report

Reference
Citation	Nahm, M., Long, A.A., Paik, S.K., Kim, S., Bae, Y.C., Broadie, K., Lee, S. (2010). The Cdc42-selective GAP Rich regulates postsynaptic development and retrograde BMP transsynaptic signaling. J. Cell Biol. 191(3): 661--675. (Export to RIS)
FlyBase ID	FBrf0212218
Publication Type	Research paper
PubMed ID	21041451
PubMed Abstract	Retrograde bone morphogenetic protein signaling mediated by the Glass bottom boat (Gbb) ligand modulates structural and functional synaptogenesis at the Drosophila melanogaster neuromuscular junction. However, the molecular mechanisms regulating postsynaptic Gbb release are poorly understood. In this study, we show that Drosophila Rich (dRich), a conserved Cdc42-selective guanosine triphosphatase-activating protein (GAP), inhibits the Cdc42-Wsp pathway to stimulate postsynaptic Gbb release. Loss of dRich causes synaptic undergrowth and strongly impairs neurotransmitter release. These presynaptic defects are rescued by targeted postsynaptic expression of wild-type dRich but not a GAP-deficient mutant. dRich inhibits the postsynaptic localization of the Cdc42 effector Wsp (Drosophila orthologue of mammalian Wiskott-Aldrich syndrome protein, WASp), and manifestation of synaptogenesis defects in drich mutants requires Wsp signaling. In addition, dRich regulates postsynaptic organization independently of Cdc42. Importantly, dRich increases Gbb release and elevates presynaptic phosphorylated Mad levels. We propose that dRich coordinates the Gbb-dependent modulation of synaptic growth and function with postsynaptic development.
DOI	10.1083/jcb.201007086
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Language of Publication	English
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Publication Type	Journal
Abbreviation	J. Cell Biol.
Title	Journal of Cell Biology
Publication Year	1966-
ISBN/ISSN	0021-9525
Data from Reference
Aberrations (2)
	Df(3L)ED4342 Df(3R)3450
Alleles (19)
	Cip4¹ Cip4^{Scer\UAS.N.T:Ivir\HA1} Dad^j1E4 gbb¹ gbb^{Scer\UAS.T:Avic\GFP} RhoGAP92B¹ RhoGAP92B² RhoGAP92B^G4993 RhoGAP92B^G6428 RhoGAP92B^{R287A.Scer\UAS} RhoGAP92B^Scer\UAS.cNa RhoGAP92B^{Scer\UAS.T:Ivir\HA1} RhoGAP92B^{ΔBAR.Scer\UAS.T:Avic\GFP} RhoGAP92B^{ΔP4.Scer\UAS.T:Ivir\HA1} Scer\GAL4^C57 Scer\GAL4^elav-C155 WASp¹ WASp^Scer\UAS.cBa wit^A12
Constructs (9)
	P{EP} P{UAS-gbb.GFP} P{UAS-HA-Cip4} P{UAS-RhoGAP92B.HA.ΔP4} P{UAS-RhoGAP92B.HA} P{UAS-RhoGAP92B.N} P{UAS-RhoGAP92B.R287A} P{UAS-RhoGAP92B.ΔBAR-GFP} P{UAS-WASp.B}
Genes (24)
	brp Cdc42 CG12378 Cip4 Csp Dad dlg1 elav gbb GluRIIA GluRIIB GluRIIC Mad Rac1 Rho1 RhoGAP92B Scer\GAL4 Surf6 T:Avic\GFP T:Ivir\HA1 WASp wit Xport α-Spec
Insertions (4)
	P{EP}RhoGAP92B^G4993 P{EP}RhoGAP92B^G6428 P{GawB}C57 P{GawB}elav^C155
Natural transposons (1)
	P-element