Reference Report

Reference
Citation	Chang, K.C., Garcia-Alvarez, G., Somers, G., Sousa-Nunes, R., Rossi, F., Lee, Y.Y., Soon, S.B., Gonzalez, C., Chia, W., Wang, H. (2010). Interplay between the Transcription Factor Zif and aPKC Regulates Neuroblast Polarity and Self-Renewal. Dev. Cell 19(5): 778--785. (Export to RIS)
FlyBase ID	FBrf0212286
Publication Type	Research paper
PubMed ID	21074726
PubMed Abstract	How a cell decides to self-renew or differentiate is a critical issue in stem cell and cancer biology. Atypical protein kinase C (aPKC) promotes self-renewal of Drosophila larval brain neural stem cells, neuroblasts. However, it is unclear how aPKC cortical polarity and protein levels are regulated. Here, we have identified a zinc-finger protein, Zif, which is required for the expression and asymmetric localization of aPKC. aPKC displays ectopic cortical localization with upregulated protein levels in dividing zif mutant neuroblasts, leading to neuroblast overproliferation. We show that Zif is a transcription factor that directly represses aPKC transcription. We further show that Zif is phosphorylated by aPKC both in vitro and in vivo. Phosphorylation of Zif by aPKC excludes it from the nucleus, leading to Zif inactivation in neuroblasts. Thus, reciprocal repression between Zif and aPKC act as a critical regulatory mechanism for establishing cell polarity and controlling neuroblast self-renewal.
DOI	10.1016/j.devcel.2010.10.007
Related Publication(s)
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Associated Information
Comments
Associated Files
Other Information
Secondary IDs
Language of Publication	English
Additional Languages of Abstract
Also Published As
Parent Publication
Publication Type	Journal
Abbreviation	Dev. Cell
Title	Developmental Cell
Publication Year	2001-
ISBN/ISSN
Data from Reference
Aberrations (1)
	Df(3R)WIN11
Alleles (7)
	aPKC^k06403 l(2)gl^{3A.Scer\UAS.P\T} Mmus\Cd8a^{Scer\UAS.T:Avic\GFP} Scer\GAL4^elav-C155 Zif^1L15 Zif^2L497 Zif^2L745
Constructs (2)
	P{UAS-mCD8::GFP.L} P{UASp-l(2)gl.3A}
Genes (24)
	aPKC ase aur baz brat Cdc42 Dap160 dpn flfl Gapdh1 Gapdh2 l(2)gl mira Mmus\Cd8a mts numb par-6 polo pon pros raps Scer\GAL4 stau Zif
Insertions (1)
	P{GawB}elav^C155