Reference Report

Reference
Citation	Sens, K.L., Zhang, S., Jin, P., Duan, R., Zhang, G., Luo, F., Parachini, L., Chen, E.H. (2010). An invasive podosome-like structure promotes fusion pore formation during myoblast fusion. J. Cell Biol. 191(5): 1013--1027. (Export to RIS)
FlyBase ID	FBrf0212349
Publication Type	Research paper
PubMed ID	21098115
PubMed Abstract	Recent studies in Drosophila have implicated actin cytoskeletal remodeling in myoblast fusion, but the cellular mechanisms underlying this process remain poorly understood. Here we show that actin polymerization occurs in an asymmetric and cell type-specific manner between a muscle founder cell and a fusion-competent myoblast (FCM). In the FCM, a dense F-actin-enriched focus forms at the site of fusion, whereas a thin sheath of F-actin is induced along the apposing founder cell membrane. The FCM-specific actin focus invades the apposing founder cell with multiple finger-like protrusions, leading to the formation of a single-channel macro fusion pore between the two muscle cells. Two actin nucleation-promoting factors of the Arp2/3 complex, WASP and Scar, are required for the formation of the F-actin foci, whereas WASP but not Scar promotes efficient foci invasion. Our studies uncover a novel invasive podosome-like structure (PLS) in a developing tissue and reveal a previously unrecognized function of PLSs in facilitating cell membrane juxtaposition and fusion.
DOI	10.1083/jcb.201006006
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Language of Publication	English
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Publication Type	Journal
Abbreviation	J. Cell Biol.
Title	Journal of Cell Biology
Publication Year	1966-
ISBN/ISSN	0021-9525
Data from Reference
Alleles (16)
	Act5C^{Scer\UAS.P\T.T:Avic\GFP} Avic\GFP^{S65T.Scer\UAS} Disc\RFP^{Scer\UAS.T:Hsap\CAAX.cSa} Ecol\lacZ^{Scer\UAS.T:SV40\nls2} Hem^J4-48 SCAR^k13811 SCAR^S196 SCAR^Scer\UAS.cSa SCAR^{Scer\UAS.T:SV5\V5} SCAR^Δ37 Scer\GAL4^kirre-rP298 Scer\GAL4^sns.PK Scer\GAL4^twi.PG Vrp1^D30 Vrp1^S1946 WASp³
Constructs (8)
	P{GAL4-twi.G} P{sns-GAL4.K} P{UAS-GFP.S65T} P{UAS-lacZ.NZ} P{UAS-mCherry.CAAX.S} P{UASp-Act5C.T:GFP} P{UAS-SCAR.S} P{UAS-SCAR.V5}
Genes (16)
	Act5C ants Avic\GFP Disc\RFP Ecol\lacZ Hem kirre lmd Mhc SCAR Scer\GAL4 sns T:Hsap\CAAX T:SV5\V5 Vrp1 WASp
Insertions (1)
	P{GAL4}rP298
Natural transposons (1)
	P-element