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Citation
Sens, K.L., Zhang, S., Jin, P., Duan, R., Zhang, G., Luo, F., Parachini, L., Chen, E.H. (2010). An invasive podosome-like structure promotes fusion pore formation during myoblast fusion.  J. Cell Biol. 191(5): 1013--1027.
FlyBase ID
FBrf0212349
Publication Type
Research paper
Abstract

Recent studies in Drosophila have implicated actin cytoskeletal remodeling in myoblast fusion, but the cellular mechanisms underlying this process remain poorly understood. Here we show that actin polymerization occurs in an asymmetric and cell type-specific manner between a muscle founder cell and a fusion-competent myoblast (FCM). In the FCM, a dense F-actin-enriched focus forms at the site of fusion, whereas a thin sheath of F-actin is induced along the apposing founder cell membrane. The FCM-specific actin focus invades the apposing founder cell with multiple finger-like protrusions, leading to the formation of a single-channel macro fusion pore between the two muscle cells. Two actin nucleation-promoting factors of the Arp2/3 complex, WASP and Scar, are required for the formation of the F-actin foci, whereas WASP but not Scar promotes efficient foci invasion. Our studies uncover a novel invasive podosome-like structure (PLS) in a developing tissue and reveal a previously unrecognized function of PLSs in facilitating cell membrane juxtaposition and fusion.

PubMed ID
PubMed Central ID
PMC2995175 (PMC) (EuropePMC)
Related Publication(s)
Note

Cell-cell fusion: a new function for invadosomes.
Sung and Weaver, 2011, Curr. Biol. 21(3): R121--R123 [FBrf0214360]

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Secondary IDs
    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    J. Cell Biol.
    Title
    Journal of Cell Biology
    Publication Year
    1966-
    ISBN/ISSN
    0021-9525
    Data From Reference