Reference Report

Reference
Citation	Lee, J., Wu, C.F. (2010). Orchestration of stepwise synaptic growth by k+ and Ca2+ channels in Drosophila. J. Neurosci. 30(47): 15821--15833. (Export to RIS)
FlyBase ID	FBrf0212405
Publication Type	Research paper
PubMed ID	21106821
PubMed Abstract	Synapse formation is tightly associated with neuronal excitability. We found striking synaptic overgrowth caused by Drosophila K(+)-channel mutations of the seizure and slowpoke genes, encoding Erg and Ca(2+)-activated large-conductance (BK) channels, respectively. These mutants display two distinct patterns of "satellite" budding from larval motor terminus synaptic boutons. Double-mutant analysis indicates that BK and Erg K(+) channels interact with separate sets of synaptic proteins to affect distinct growth steps. Post-synaptic L-type Ca(2+) channels, Dmca1D, and PSD-95-like scaffold protein, Discs large, are required for satellite budding induced by slowpoke and seizure mutations. Pre-synaptic cacophony Ca(2+) channels and the NCAM-like adhesion molecule, Fasciclin II, take part in a maturation step that is partially arrested by seizure mutations. Importantly, slowpoke and seizure satellites were both suppressed by rutabaga mutations that disrupt Ca(2+)/CaM-dependent adenylyl cyclase, demonstrating a convergence of K(+) channels of different functional categories in regulation of excitability-dependent Ca(2+) influx for triggering cAMP-mediated growth plasticity.
DOI	10.1523/JNEUROSCI.3448-10.2010
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Language of Publication	English
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Publication Type	Journal
Abbreviation	J. Neurosci.
Title	Journal of Neuroscience
Publication Year	1981-
ISBN/ISSN	0270-6474 1529-2401
Data from Reference
Aberrations (1)
	Df(1)N71
Alleles (26)
	cac^NT27 cac^S Ca-α1D^AR66 dlg1⁷ dlg1^{WT.Scer\UAS.T:Avic\GFP-EGFP} dnc^M14 dnc^Scer\UAS.cCa Fas2^e76 Fas2^EB112 mle^nap-ts1 rut¹ rut¹⁰⁸⁴ Scer\GAL4^C164 Scer\GAL4^elav-C155 Scer\GAL4^Mef2.247 sei¹ sei² sei^GD2196 sei^KK105733 sei^ts2.Scer\UAS slo¹ slo⁴ slo⁹⁸ slo^GD244 slo^KK108671 T:Avic\GFP^EGFP
Constructs (8)
	P{GD244} P{GD2196} P{KK105733} P{KK108671} P{Mef2-GAL4.247} P{UAS-dlg1-EGFP} P{UAS-dnc.C} P{UAS-sei.ts2}
Genes (12)
	brp cac Ca-α1D dlg1 dnc Fas2 GluRIIC mle rut Scer\GAL4 sei slo
Insertions (2)
	P{GawB}C164 P{GawB}elav^C155
Natural transposons (1)
	P-element