Reference Report

Reference
Citation	Padash-Barmchi, M., Browne, K., Sturgeon, K., Jusiak, B., Auld, V.J. (2010). Control of Gliotactin localization and levels by tyrosine phosphorylation and endocytosis is necessary for survival of polarized epithelia. J. Cell Sci. 123(23): 4052--4062. (Export to RIS)
FlyBase ID	FBrf0212409
Publication Type	Research paper
PubMed ID	21045109
PubMed Abstract	The tricellular junction (TCJ) forms at the convergence of bicellular junctions from three adjacent cells in polarized epithelia and is necessary for maintaining the transepithelial barrier. In the fruitfly Drosophila, the TCJ is generated at the meeting point of bicellular septate junctions. Gliotactin was the first identified component of the TCJ and is necessary for TCJ and septate junction development. Gliotactin is a member of the neuroligin family and associates with the PDZ protein discs large. Beyond this interaction, little is known about the mechanisms underlying Gliotactin localization and function at the TCJ. In this study, we show that Gliotactin is phosphorylated at conserved tyrosine residues, a process necessary for endocytosis and targeting to late endosomes and lysosomes for degradation. Regulation of Gliotactin levels through phosphorylation and endocytosis is necessary as overexpression results in displacement of Gliotactin away from the TCJ throughout the septate junction domain. Excessive Gliotactin in polarized epithelia leads to delamination, paired with subsequent migration, and apoptosis. The apoptosis and the resulting compensatory proliferation resulting from high levels of Gliotactin are mediated by the Drosophila JNK pathway. Therefore, Gliotactin levels within the cell membrane are regulated to ensure correct protein localization and cell survival.
DOI	10.1242/jcs.066605
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Language of Publication	English
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Publication Type	Journal
Abbreviation	J. Cell Sci.
Title	Journal of Cell Science
Publication Year	1966-
ISBN/ISSN	0021-9533
Data from Reference
Alleles (14)
	BacA\p35^Scer\UAS.cHa bsk^DN.Scer\UAS Ecol\lacZ^puc-A251.1F3 Ecol\lacZ^wg-en11 Gli^AE2Δ45 Gli^DD.Scer\UAS Gli^FF.Scer\UAS Gli^Scer\UAS.cPa Mmus\Cd8a^{Scer\UAS.T:Avic\GFP} Nrx-IV^EP604 puc^JK0 Rab5^{S43N.Scer\UAS.P\T.T:Avic\GFP-YFP} Scer\GAL4^ap-md544 Scer\GAL4^da.G32
Constructs (8)
	P{GAL4-da.G32} P{UAS-bsk.DN} P{UAS-Gli.DD} P{UAS-Gli.FF} P{UAS-Gli.P} P{UAS-mCD8::GFP.L} P{UAS-p35.H} P{UASp-YFP.Rab5.S43N}
Genes (14)
	BacA\p35 bsk dlg1 Ecol\lacZ Gli Hrs Mad Mmp1 Mmus\Cd8a Nrx-IV puc Rab5 Scer\GAL4 shg
Insertions (4)
	P{en1}wg^en11 P{EP}Nrx-IV^EP604 P{GawB}ap^md544 P{lArB}puc^A251.1F3
Natural transposons (1)
	P-element