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Tsurudome, K., Tsang, K., Liao, E.H., Ball, R., Penney, J., Yang, J.S., Elazzouzi, F., He, T., Chishti, A., Lnenicka, G., Lai, E.C., Haghighi, A.P. (2010). The Drosophila miR-310 Cluster Negatively Regulates Synaptic Strength at the Neuromuscular Junction.  Neuron 68(5): 879--893.
FlyBase ID
FBrf0212495
Publication Type
Research paper
Abstract
Emerging data implicate microRNAs (miRNAs) in the regulation of synaptic structure and function, but we know little about their role in the regulation of neurotransmission in presynaptic neurons. Here, we demonstrate that the miR-310-313 cluster is required for normal synaptic transmission at the Drosophila larval neuromuscular junction. Loss of miR-310-313 cluster leads to a significant enhancement of neurotransmitter release, which can be rescued with temporally restricted expression of mir-310-313 in larval presynaptic neurons. Kinesin family member, Khc-73 is a functional target for miR-310-313 as its expression is increased in mir-310-313 mutants and reducing it restores normal synaptic function. Cluster mutants show an increase in the active zone protein Bruchpilot accompanied by an increase in electron dense T bars. Finally, we show that repression of Khc-73 by miR-310-313 cluster influences the establishment of normal synaptic homeostasis. Our findings establish a role for miRNAs in the regulation of neurotransmitter release.
PubMed ID
PubMed Central ID
PMC3034365 (PMC) (EuropePMC)
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Secondary IDs
    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    Neuron
    Title
    Neuron
    Publication Year
    1988-
    ISBN/ISSN
    0896-6273
    Data From Reference