Reference Report

Reference
Citation	Tsurudome, K., Tsang, K., Liao, E.H., Ball, R., Penney, J., Yang, J.S., Elazzouzi, F., He, T., Chishti, A., Lnenicka, G., Lai, E.C., Haghighi, A.P. (2010). The Drosophila miR-310 Cluster Negatively Regulates Synaptic Strength at the Neuromuscular Junction. Neuron 68(5): 879--893. (Export to RIS)
FlyBase ID	FBrf0212495
Publication Type	Research paper
PubMed ID	21145002
PubMed Abstract	Emerging data implicate microRNAs (miRNAs) in the regulation of synaptic structure and function, but we know little about their role in the regulation of neurotransmission in presynaptic neurons. Here, we demonstrate that the miR-310-313 cluster is required for normal synaptic transmission at the Drosophila larval neuromuscular junction. Loss of miR-310-313 cluster leads to a significant enhancement of neurotransmitter release, which can be rescued with temporally restricted expression of mir-310-313 in larval presynaptic neurons. Kinesin family member, Khc-73 is a functional target for miR-310-313 as its expression is increased in mir-310-313 mutants and reducing it restores normal synaptic function. Cluster mutants show an increase in the active zone protein Bruchpilot accompanied by an increase in electron dense T bars. Finally, we show that repression of Khc-73 by miR-310-313 cluster influences the establishment of normal synaptic homeostasis. Our findings establish a role for miRNAs in the regulation of neurotransmitter release.
DOI	10.1016/j.neuron.2010.11.016
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Secondary IDs
Language of Publication	English
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Parent Publication
Publication Type	Journal
Abbreviation	Neuron
Title	Neuron
Publication Year	1988-
ISBN/ISSN	0896-6273
Data from Reference
Aberrations (4)
	Df(2L)cl-h4 Df(2R)Exel6070 Df(2R)Exel6285 Df(2R)KT40
Alleles (26)
	brp⁶⁹ brp^{Scer\UAS.T:Avic\GFP} cac^HC129 CrebA⁰³⁵⁷⁶ Dcr-2^Scer\UAS.cDa GluRIIA^SP16 GSd033^KT2 Khc-73^{dsRNA.Scer\UAS} Khc-73^{ex14.Scer\UAS.T:Ivir\HA1} Khc-73^{Scer\UAS.T:Ivir\HA1.3UTR} Khc-73^{Scer\UAS.T:Ivir\HA1} lap^KG06751 mir-310^{Scer\UAS.cluster} mir-310^Scer\UAS.cTb mir-311^{Scer\UAS.cluster} mir-312^{Scer\UAS.cluster} mir-313^{Scer\UAS.cluster} mir-313^Scer\UAS.cTb Mmus\Cd8a^{Scer\UAS.T:Avic\GFP} Scer\GAL4^BG380 Scer\GAL4^{elav.Switch.PO} Scer\GAL4^NP4255 Scer\GAL4^NP5941 Scer\GAL4^Rapgap1-OK6 Scer\GAL4^unspecified T:Disc\RFP^DsRed2
Constructs (12)
	P{elav-Switch.O} P{GAL4} P{UAS-brp.GFP} P{UAS-Dcr-2.D} P{UAS-Khc-73.HA.3UTR} P{UAS-Khc-73.HA.ex14} P{UAS-Khc-73.HA} P{UAS-Khc-73.RNAi} P{UAS-mCD8::GFP.L} P{UAS-mir-310.cluster.DsRed} P{UAS-mir-310} P{UAS-mir-313}
Genes (19)
	brp cac CrebA Dcr-2 dlg1 GluRIIA GluRIIC GSd033 Khc-73 lap mir-306 mir-310 mir-311 mir-312 mir-313 Mmus\Cd8a Scer\GAL4 Syt1 VGlut
Insertions (7)
	P{GAL4}BG380 P{GawB}NP4255 P{GawB}NP5941 P{GawB}OK6 P{GSV1}GSd033^GSd033 P{PZ}CrebA⁰³⁵⁷⁶ P{SUPor-P}lap^KG06751
Natural transposons (1)
	P-element