Reference Report

Reference
Citation	Furrer, M., Balbi, M., Albarca-Aguilera, M., Gallant, M., Herr, W., Gallant, P. (2010). Drosophila Myc Interacts with Host Cell Factor (dHCF) to Activate Transcription and Control Growth. J. Biol. Chem. 285(51): 39623--39636. (Export to RIS)
FlyBase ID	FBrf0212501
Publication Type	Research paper
PubMed ID	20937797
PubMed Abstract	The Myc proto-oncoproteins are transcription factors that recognize numerous target genes through hexameric DNA sequences called E-boxes. The mechanism by which they then activate the expression of these targets is still under debate. Here, we use an RNAi screen in Drosophila S2 cells to identify Drosophila host cell factor (dHCF) as a novel co-factor for Myc that is functionally required for the activation of a Myc-dependent reporter construct. dHCF is also essential for the full activation of endogenous Myc target genes in S2 cells, and for the ability of Myc to promote growth in vivo. Myc and dHCF physically interact, and they colocalize on common target genes. Furthermore, down-regulation of dHCF-associated histone acetyltransferase and histone methyltransferase complexes in vivo interferes with the Myc biological activities. We therefore propose that dHCF recruits such chromatin-modifying complexes and thereby contributes to the expression of Myc targets and hence to the execution of Myc biological activities.
DOI	10.1074/jbc.M110.140467
Related Publication(s)
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Associated Information
Comments
Associated Files
Other Information
Secondary IDs
Language of Publication	English
Additional Languages of Abstract
Also Published As
Parent Publication
Publication Type	Journal
Abbreviation	J. Biol. Chem.
Title	Journal of Biological Chemistry
Publication Year	1905-
ISBN/ISSN	0021-9258
Data from Reference
Alleles (13)
	ash2^GD1374 dm^P0 dm^Scer\UAS.cZa Gcn5^GD11218 Hcf^{dsRNA.IR-2.Scer\UAS} Hcf^{dsRNA.IR-3.Scer\UAS} Hcf^GD4353 Hcf^{Scer\UAS.T:Zzzz\FLAG} Scer\GAL4^Act5C.PP Scer\GAL4^ap-md544 Scer\GAL4^GMR.PF Scer\GAL4^sca-109-68 Scer\GAL4^αTub84B.PBb
Constructs (8)
	P{GAL4-ninaE.GMR} P{GD1374} P{GD4353} P{GD11218} P{UAS-dm.Z} P{UAS-Hcf.Flag} P{UAS-Hcf.IR-2} P{UAS-Hcf.IR-3}
Genes (55)
	Ada2a ash1 ash2 Atac1 Atac2 Atac3 CG1234 CG1542 CG1785 CG4038 CG4364 CG5033 CG5728 CG6712 CG7845 CG8939 CG9799 CG10341 CG11660 CG15019 CG30349 Chrac-14 D12 dik dm Dpy-30L1 Fib FK506-bp1 Gcn5 Hcf hoip l(2)09851 l(2)k09022 mbm Mnn1 Mocs2 NC2β nclb NHP2 Nnp-1 nop5 Nop56 Nopp140 pit Pka-C1 Rbbp5 RpLP0-like Rrp46 Scer\GAL4 Set1 Sgf29 Sin3A trr trx wds
Insertions (2)
	P{GawB}ap^md544 P{GawB}sca^109-68
Natural transposons (1)
	P-element