Reference Report

Reference
Citation	Dahdal, D., Reeves, D.C., Ruben, M., Akabas, M.H., Blau, J. (2010). Drosophila Pacemaker Neurons Require G Protein Signaling and GABAergic Inputs to Generate Twenty-Four Hour Behavioral Rhythms. Neuron 68(5): 964--977. (Export to RIS)
FlyBase ID	FBrf0212523
Publication Type	Research paper
PubMed ID	21145008
PubMed Abstract	Intercellular signaling is important for accurate circadian rhythms. In Drosophila, the small ventral lateral neurons (s-LN(v)s) are the dominant pacemaker neurons and set the pace of most other clock neurons in constant darkness. Here we show that two distinct G protein signaling pathways are required in LN(v)s for 24 hr rhythms. Reducing signaling in LN(v)s via the G alpha subunit Gs, which signals via cAMP, or via the G alpha subunit Go, which we show signals via Phospholipase 21c, lengthens the period of behavioral rhythms. In contrast, constitutive Gs or Go signaling makes most flies arrhythmic. Using dissociated LN(v)s in culture, we found that Go and the metabotropic GABA(B)-R3 receptor are required for the inhibitory effects of GABA on LN(v)s and that reduced GABA(B)-R3 expression in vivo lengthens period. Although no clock neurons produce GABA, hyperexciting GABAergic neurons disrupts behavioral rhythms and s-LN(v) molecular clocks. Therefore, s-LN(v)s require GABAergic inputs for 24 hr rhythms.
DOI	10.1016/j.neuron.2010.11.017
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Neuron
Title	Neuron
Publication Year	1988-
ISBN/ISSN	0896-6273
Data from Reference
Alleles (22)
	Avic\GFP^{GCaMP1.6.Scer\UAS} Bhal\NaChBac^Scer\UAS.cNa Bper\ptxA^act.Scer\UAS Disc\RFP^Pdf.PR dnc^Scer\UAS.cCa GABA-B-R2^GD699 GABA-B-R3^GD578 Gnf1^GD4455 Gαo^GDP.Scer\UAS Gαo^GTP.Scer\UAS Gαo^{Scer\FRT.GDP.Scer\UAS} Gαo^{Scer\FRT.GTP.Scer\UAS} Gαs^{dsRNA.Scer\UAS} Gαs^{Q215L.Scer\UAS} Gαs^Scer\UAS.cCa Mmus\Cd8a^{Scer\UAS.T:Avic\GFP} Pde8^EY10946 Plc21C^GD11359 Scer\GAL4^elav-C155 Scer\GAL4^Gad1.PS Scer\GAL4^P0.5.Pdf Scer\GAL4^tim.Scer\UAS
Constructs (20)
	P{Gad1-GAL4.S} P{GD578} P{GD699} P{GD4455} P{GD11359} P{Pdf-GAL4.P0.5} P{Pdf-RFP.R} P{UAS(FRT.w⁺)G-oα47A.GDP} P{UAS(FRT.w⁺)G-oα47A.GTP} P{UAS(-FRT)G-oα47A.GDP} P{UAS(-FRT)G-oα47A.GTP} P{UAS-dnc.C} P{UAS-G-CaMP.1.6} P{UAS-G-sα60A.C} P{UAS-G-sα60A.Q215L} P{UAS-G-sα60A.RNAi} P{UAS-mCD8::GFP.L} P{UAS-NaChBac} P{UAS-PTX.act} P{UAS-tim-GAL4}
Genes (15)
	Bhal\NaChBac Bper\ptxA Disc\RFP dnc GABA-B-R2 GABA-B-R3 Gad1 Gnf1 Gαo Gαs Mmus\Cd8a Pde8 Plc21C Scer\GAL4 vri
Insertions (2)
	P{EPgy2}Pde8^EY10946 P{GawB}elav^C155
Natural transposons (1)
	P-element
Transcripts (1)
	Scer\GAL4^Gad1.PSRA