Reference Report

Reference
Citation	Zobeck, K.L., Buckley, M.S., Zipfel, W.R., Lis, J.T. (2010). Recruitment timing and dynamics of transcription factors at the hsp70 Loci in living cells. Mol. Cell 40(6): 965--975. (Export to RIS)
FlyBase ID	FBrf0212598
Publication Type	Research paper
PubMed ID	21172661
PubMed Abstract	Chromatin immunoprecipitation (ChIP) studies provide snapshots of factors on chromatin in cell populations. Here, we use live-cell imaging to examine at high temporal resolution the recruitment and dynamics of transcription factors to the inducible Hsp70 loci in individual Drosophila salivary gland nuclei. Recruitment of the master regulator, HSF, is first detected within 20 s of gene activation; the timing of its recruitment resolves from RNA polymerase II and P-TEFb, and these factors resolve from Spt6 and Topo I. Remarkably, the recruitment of each factor is highly synchronous between different cells. In addition, fluorescence recovery after photobleaching (FRAP) analyses show that the entry and exit of multiple factors are progressively constrained upon gene activation, suggesting the gradual formation of a transcription compartment. Furthermore, we demonstrate that poly(ADP-ribose) (PAR) polymerase activity is required to maintain the transcription compartment. We propose that PAR polymers locally retain factors in a transcription compartment.
DOI	10.1016/j.molcel.2010.11.022
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Supplementary material	Supplemental Information. [FBrf0212918]
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Mol. Cell
Title	Molecular Cell
Publication Year	1997-
ISBN/ISSN	1097-2765 1097-4164
Data from Reference
Alleles (8)
	Avic\GFP^Spt6-CA07692 Avic\GFP^Top1-CC01414 CycT^{Scer\UAS.T:Disc\RFP-mRFP} His2B^{Scer\UAS.cZa.T:Disc\RFP-mRFP} Hsf^{Scer\UAS.T:Avic\GFP-EGFP} Parp^{Scer\UAS.T:Avic\GFP-EGFP} RpII33^{Scer\UAS.T:Avic\GFP-EGFP} RpII33^{Scer\UAS.T:Disc\RFP-mRFP}
Constructs (6)
	P{UAS-HSF-EGFP} P{UAS-mRFP-CycT} P{UAS-mRFP-His2B.Z} P{UAS-mRFP-Rpb3} P{UAS-Parp-eGFP} P{UAS-RpII33-EGFP}
Genes (14)
	Avic\GFP CycT His2B Hsf Hsp70Aa Hsp70Ab Hsp70Ba Hsp70Bb Hsp70Bbb Hsp70Bc Parp RpII33 Spt6 Top1
Insertions (2)
	P{PTT-GA}Spt6^CA07692 P{PTT-GC}Top1^CC01414